小白菊内酯抑制肾癌786-O细胞增殖及对细胞周期表达的影响  

Effects of parthenolide on proliferation inhibiting and cell cycle of human renal cancer 786-O cells

作　　者：董莉[1] 石玮[2] 孙延庆[1] 李伟华[1] Dong Li;Shi Wei;Sun Yanqing(Department of Central Laboratory,the People＇s Hospital of C.ansu Province,Lanzhou 730000,China;Department of Urology,the Second Hospital of Lanzhou University,Lanzhou 730030,China)

机构地区：[1]甘肃省人民医院中心实验室,甘肃兰州730000 [2]兰州大学第二医院泌尿外科甘肃省泌尿疾病临床医学中心研究重点实验室,甘肃兰州730030

出　　处：《国际泌尿系统杂志》2018年第6期937-940,共4页International Journal of Urology and Nephrology

摘　　要：目的 研究小白菊内酯对人肾癌786-O细胞增殖和凋亡的影响及其可能存在机制.方法 应用不同浓度的小白菊内酯作用于786-O细胞,使用MTT法观察786-O细胞在增殖方面的变化,流式细胞术检测小白菊内酯对细胞周期和细胞凋亡的影响,荧光定量PCR法检测Bcl-2和Bax基因的变化,蛋白印迹法检测Bax和Bcl-2蛋白的变化.结果 小白菊内酯对786-O细胞增殖具有明显抑制作用,并呈显著浓度依赖性和时间依赖性.小白菊内酯可以诱导786-O细胞发生凋亡,并可以改变786-O细胞在不同细胞周期的分布,包括G1期细胞所占百分比升高,S期细胞所占百分比降低.随着小白菊内酯作用浓度的增加,可以使CyclinD1表达减少,并有显著的浓度依赖性.同时,在基因和蛋白水平上,小白菊内酯能抑制Bcl-2的表达而促进Bax的表达.结论 小白菊内酯能显著抑制786-O细胞增殖,诱导786-O细胞发生凋亡,其作用机制与可能与抑制786-O细胞周期蛋白CyclinDl表达,调节Bcl-2、Bax表达变化有关.Objective To explore the effects of parthenolide on growth inhibition,cell aoptosis and molecular mechanism of human 786-O kidney cancer cells.Methods The inhibitory effect of parthenolide on human786-O cells was determined by MTT assay.The cell apoptosis and the cell cycle were analyzed by flow cytometry.Bax and the gene change of Bax and Bcl-2 were analyzed by fluorescence quantitative PCR,Bax and Bcl-2 family proteins were analyzed by Westerm blot.Results Parthenolide inhibited the proliferation of 786-O cells in a dose-dependent and time-dependent manner.As showed by flow cytometry,parthenolide treatment of the 786-O cells led to significant G1-phase cell cycle arrest in a dose-dependent manner and a decrease in cell population in the S-phase,whereas the population of cells in G2/M phase did not chang significantly.Flow cytometIy also showed a dose-dependent increase in 786-O cells apoptosis by parthenolide treatment.Westem blot analysis indicated a down-regulation of phosphorylated CyclinD1 in a dose-dependent manner in 786-O cells by parthenolide treatment.In the gene and protein level,parthenolide treatment to 786-O cell lines resulted a significant reduction in Bcl-2 expression but an increase in Bax protein in a dose-dependent manner.Conclusions Parthenolide treatment can 1 ead to a significant dose-dependent and time-dependent inhibition in the growth and an increase in apoptosis of human 786-O Kidney cancer cells.The mechanism of action of parthenolide may be related to inhibition of CyclinD1 activation and regulation of Bcl-2 and Bax expression in human 786-O Kidney cancer cells.

关 键 词：肾肿瘤 菊蒿 细胞增殖 细胞凋亡 细胞周期 

分 类 号：R737.11[医药卫生—肿瘤]