芦荟大黄素与顺铂对乳腺癌MDA-MB-231细胞Bcl-2及Bcl-xL表达水平的影响  

Influence of aloe emodin and cisplatin on expression level of Bcl-2 and Bcl-xL in breast cancer MDA-MB-231 cells

作　　者：孙玮[1] Sun Wei(Shanxi Cancer Hospital,Affiliated Tumor Hospital of Shanxi Medical University,Taiyuan 030002,China)

机构地区：[1]山西省肿瘤医院山西医科大学附属肿瘤医院,太原030002

出　　处：《中国实用医刊》2018年第21期112-115,共4页Chinese Journal of Practical Medicine

摘　　要：目的 探讨芦荟大黄素与顺铂对乳腺癌MDA-MB-231细胞的Bcl-2及Bcl-xL表达水平的影响.方法 培养MDA-MB-231细胞,随机分为空白对照组、芦荟大黄素组、顺铂组和联合用药组.芦荟大黄素组给予30 mol/L芦荟大黄素处理,顺铂组给予12 mol/L顺铂处理,联合用药组给予30 mol/L芦荟大黄素＋12 mol/L顺铂处理,采用MTT法检测细胞增殖,流式细胞仪检测细胞凋亡及细胞周期,Western blot检测Bcl-2及Bcl-xL蛋白表达.结果 联合用药组对MDA-MB-231细胞增殖的抑制率为（68.48±8.75）％,高于芦荟大黄素组[（54.28±8.39）％]与顺铂组[（52.04±9.11）％],P〈0.05;联合用药组G0/G1细胞比例为（87.91±9.10）％,高于芦荟大黄素组[（64.21±8.45）％]、顺铂组[（67.08±9.82）％]和空白对照组[（56.38±7.93）％],比较差异有统计学意义（P〈0.05）;芦荟大黄素组与顺铂组G0/G1（64.21±8.45、67.08±9.82）细胞比例均高于空白对照组（56.38±7.93）,P〈0.05.联合用药组细胞凋亡率为（17.38±3.20）％,高于芦荟大黄素组的（7.84±0.83）％、顺铂组的（6.90±1.10）％和空白对照组的（0.63±0.07）％,P〈0.05;芦荟大黄素组与顺铂组细胞凋亡率均高于空白对照组（P〈0.05）.空白对照组Bcl-2和Bcl-xL蛋白相对表达量分别为1.02±0.16、1.01±0.17,均高于芦荟大黄素组（0.67±0.10、0.52±0.11）、顺铂组（0.70±0.11、0.50±0.09）和联合用药组（0.64±0.13、0.13±0.04）,P〈0.05;联合用药组Bcl-xL蛋白相对表达量低于芦荟大黄素组和顺铂组（P〈0.05）.联合用药组MDA-MB-231纤连蛋白和层粘连蛋白抑制率分别为28.10±4.10、30.01±9.82,高于芦荟大黄素组（13.28±3.10、14.28±6.72）和顺铂组（14.50±3.32、15.10±7.08）,P〈0.05.联合用药组MDA-MB-231细胞侵袭能力抑制率为（54.27±8.83）％,高于芦荟大黄素组的（28.10±8.22）％和顺铂组的（30.10±9.10）％,P〈0.05.结论 芦荟大黄素与顺铂能有效抑制乳腺癌Objective To investigate the effects of aloe emodin and cisplatin on the expression of Bcl-2 and Bcl-xL in breast cancer MDA-MB-231 cells.Methods Cultured MDA-MB-231 cells were randomly divided into blank control group , aloe emodin group , cisplatin group and combined drug group . Aloe emodin group was given 30 mol/L aloe emodin, while cisplatin group was given 12 mol/L cisplatin, and combined drug group was given 30 mol/L aloe emodin ＋12 mol/L cisplatin.Cell proliferation was detected by MTT method .Cell apoptosis and cell cycle were detected by flow cytometry .The expression of Bcl-2 and Bcl-xL protein was detected by Western blot .Results The inhibitory rate of MDA-MB-231 cell proliferation in combined drug group was （ 68.48 ±8.75 ）％, which was significantly higher than that in aloe emodin group [（54.28 ±8.39）％] and cisplatin group [（52.04 ±9.11）％], P〈0.05. The proportion of G 0/G1 cells in combined drug group was [ （ 87.91 ± 9.10 ）％] , which was significantly higher than that in aloe emodin group [ （ 64.21 ±8.45 ）％] , cisplatin group [ （ 67.08 ± 9.82）％] and blank control group [（56.38 ±7.93）％], P〈0.05.The proportion of G0/G1 cells in＆nbsp;aloe emodin group and cisplatin group was significantly higher than that of blank control group （ P〈0.05）.The apoptosis rate of combined drug group was [（17.38 ±3.20）％], which was significantly higher than that of aloe emodin group （ 7.84 ±0.83 ）％] , cisplatin group [ （ 6.90 ±1.10 ）％] and blank control group [ （ 0.63 ±0.07 ）％] , P〈0.05 .The apoptosis rate of aloe emodin group and cisplatin group was significantly higher than that of blank control group （ P〈0.05 ） .The relative expression levels of Bcl-2 and Bcl-xL in blank control group were 1.02 ±0.16 and 1.01 ±0.17 , which were significantly higher than those of aloe emodin group （ 0.67 ±0.10 ,0.52 ±0.11 ） , cisplatin group （0.70 ±0.11, 0.50 ±0.09） and combined drug group （0.64 ±0.13, 0.13 ±0.04）, P〈0.0

关 键 词：芦荟大黄素 顺铂 MDA-MB-231细胞 BCL-2 BCL-XL 

分 类 号：R737.9[医药卫生—肿瘤]