长链非编码RNA LINC00087在神经胶质瘤中的表达及意义  

The expression and significance of long non-coding RNA LINC00087 in glioma

作　　者：胡珊珊 汤深凤 牛朝诗 Hu Shan-shah;Tang Shenfeng;Niu Chaoshi(Anhui Provincial Stereotactic Neurosurgical Institute,Department of Neurosurgery of The First Affiliated Hospital of USTC,Division of Life Sciences and Medicine,University of Science and Technology of China,Anhui Key Laboratory of Brain Function and Diseases,Hefei,Anhui,230001,P.R.China.)

机构地区：[1]安徽省脑立体定向神经外科研究所,中国科学技术大学附属第一医院神经外科,脑功能与脑疾病安徽省重点实验室,合肥230001

出　　处：《立体定向和功能性神经外科杂志》2018年第3期133-136,共4页Chinese Journal of Stereotactic and Functional Neurosurgery

基　　金：安徽省科技攻关项目(编号:1604a0802069);中央引导地方科技发展专项项目(编号:2017070802D144);脑功能与脑疾病安徽省重点实验室绩效考核补助项目(编号:1606c08235);国家自然科学基金项目(编号:81172407)

摘　　要：目的探索长链非编码RNA LINC00087在神经胶质瘤病人组织中的表达及意义。方法用生物信息学方法分析国际数据库GEO中收录的GSE58276中差异表达的长链非编码RNA;RT-PCR的方法证明LINC00087在胶质瘤细胞系U87中的表达;实时荧光定量PCR分析26例神经胶质瘤组织(WHO Ⅰ～Ⅱ级13例,Ⅲ～Ⅳ级13例)和14例正常脑组织中长链非编码RNA LINC00087的表达。结果长链非编码RNA LINC00087在神经胶质瘤中显著高表达,且与WHO分级相关(P<0.0001)。结论长链非编码RNA LINC00087在神经胶质瘤样本中高表达,可能成为神经胶质瘤重要的预后指标和新的治疗靶点。Objective To discuss the expression and significance of lncRNA LINC00087 in glioma.Methods Differential expressed LncRNAs were identified in international Database GEO（GSE58276）with bioinformatic analysis;the LINC00087 expression was detected by using RTPCR in U87 cell lines;the expression level of LINC00087 between 26 glioma tissues（Grade Ⅰ-Ⅱ in 13 cases and Grade Ⅲ-Ⅳ in 13 cases,according to WHO criteria）and 14 normal brain tissues was detected by using real-time fluorescence quantitative PCR.Results LINC00087 expression in the glioma tissues was obviously higher as compared with the normal brain tissues,and the expression was significantly correlated to grading of the tumors（P〈0.0001）.Conclusion The high expression of LINC00087 in glioma tissues suggests LINC00087 may be an important prognostic marker and a new therapeutic target for glioma.

关 键 词：长链非编码RNA 神经胶质瘤 

分 类 号：R730.264[医药卫生—肿瘤]