钩藤碱对培养的大鼠海马星形胶质细胞氧-葡萄糖剥夺后兴奋性氨基酸转运体2和NMDA受体2B mRNA表达的影响  

Effect of rhynchophylline on the mRNA expression of excitatory amino acid transporter 2 and NMDA receptor 2B after oxygen-glucose deprivation in cultured rat hippocampal astrocytes

作　　者：陈艳红 楼小亮[2] 周征成[2] 胡学勇 席秋江[2] 杨宏武[2] 肖丽华[2] 李欣弘[2] 李晓萍[2] 范华[2] Chen Yanhong;Lou Xiaoliang;Zhou Zhengcheng;Hu Xueyong;Xi Qiujiang;Yang Hongwu;Xiao Lihua;Li Xinhong;Li Xiaoping;Fan Hua(Department of Neurology,Ji＇an Hospital of Shanghai Oriental Hospital,Ji＇an 343600,China;Department of Neurology,the Fourth Affiliated Hospital of Nanchang University,Nanchang 330000,China)

机构地区：[1]上海市东方医院吉安医院神经内科,吉安343600 [2]南昌大学第四附属医院神经内科,330000

出　　处：《国际脑血管病杂志》2018年第9期684-688,共5页International Journal of Cerebrovascular Diseases

基　　金：江西省自然科学基金（20114BA13205076）;江西省科技计划项目（20151BBG70201）;江西省教育厅科学技术项目（14089）;江西省卫生计生委中医药重点科研课题（2016206）

摘　　要：目的 探讨钩藤碱对氧-葡萄糖剥夺后星形胶质细胞兴奋性氨基酸转运体2（excitatory amino acid transporter 2,EAAT2）和N-甲基-D-天冬氨酸受体2B（N-methyl-D-aspartic acid receptor 2B,NR2B）mRNA表达的影响.方法 将传代培养至第3代的海马区星形胶质细胞接种至6孔板,待细胞贴壁并长出突起后分为空白对照组、缺血缺氧组以及小剂量（0.02 mg/ml）和大剂量（0.2 mg/ml）钩藤碱组.提取各组总RNA,采用实时荧光定量聚合酶链反应检测各组星形胶质细胞EAAT2和NR2B mRNA表达水平.结果 与空白对照组比较,缺血缺氧组星形胶质细胞NR2B和EAAT2 mRNA表达水平均显著增高（P均〈0.05）.小剂量钩藤碱组NR2B和EAAT2 mRNA表达水平较缺血缺氧组降低,但差异无统计学意义.大剂量钩藤碱组NR2B和EAAT2 mRNA表达水平显著降低,与缺血缺氧组和小剂量钩藤碱组相比差异均有统计学意义（P均〈0.05）.结论 体外培养的海马区星形胶质细胞缺血缺氧后EAAT2和NR2B mRNA表达显著增高,钩藤碱干预可呈浓度依赖性方式显著下调其表达.Objective To investigate the effect of rhynchophylline on mRNA expression of excitatory amino acid transporter 2 （EAAT2 ） and N-methyl-D-aspartic acid receptor 2B （NR2B） after astrocyte oxygen-glucose deprivation. Methods The subcultured third generation astrocytes from the hippocampus were inoculated into 6-well plates, and they were divided into blank control group, hypoxia-ischemia group,low-dose rhynchophylline group （0. 02 mg/ml） and high-dose rhynchophylline group （0. 2 mg/ml） after the cells were attached to the wall and grew out protrusion. The total RNAs in each group were extracted.Real-time fluorescence quantitative polymerase chain reaction was used to detect the expression levels of EAAT2 and NR2B mRNA in astrocytes of each group. Results Compared with the blank control group, the expression levels of NR2B and EAAT2 mRNA in astrocytes of the ischemia-hypoxia group were significantly higher （all P 〈 0. 05 ）. The expression levels of NR2B and EAAT2 mRNA in the low-dose rhynchophylline group were lower than those in ischemia-hypoxia group, but there was no significant difference. The expression levels of NR2B and EAAT2 mRNA in the high-dose rhynchophylline group were significantly lower than the ischemia-hypoxia group and the low-dose rhynchophylline group （all P 〈 0. 05）.Conclusion The expression of EAAT2 and NR2B mRNA in astrocytes of hippocampus cultured in vitro was significantly increased after ischemia and hypoxia, and rhynchophylline intervention could significantly reduce its expression in a concentration dependent manner.

关 键 词：星形胶质细胞 细胞 培养的 钩藤碱 葡萄糖 缺氧 氧 兴奋性氨基酸转运蛋白2 受体 N-甲基-D-天冬氨酸 基因表达 大鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学]