Experimental study of Quercetin self-nanoemulsifying drug delivery system in inhibiting SMMC-7721 cells  

作　　者：Yang Sun Yan-Xin Che Bo-Yan Wu Yang Sun;Yan-Xin Che;Bo-Yan Wu(Heilongjiang University of Traditional Chinese Medicine, Haerbin, Heilongjiang, China;Daqing Second Hospital, Daqing, Heilongjiang, China)

机构地区：[1]Heilongjiang University of Traditional Chinese Medicine, Haerbin, Heilongjiang, China [2]Daqing Second Hospital, Daqing, Heilongjiang, China

出　　处：《TMR Cancer》2018年第4期99-104,共6页TMR肿瘤

摘　　要：Objective： To study the mechanism of inhibition of SMMC-7721 liver cancer cells by quercetin self-nanoemulsifying drug delivery system （Q-SNEDDS） in vitro. Methods： The inhibitory effect of Q-SNEDDS on hepatoma cells was detected by MTT assay. The effect of Q-SNEDDS on apoptosis of hepatoma cells was detected by flow cytometry. The changes of Q-SNEDDS after hepatoma cells were observed by fluorescence microscopy. The effect of Q-SNEDDS on the expression of signal tra_nsduction and activator of transcription 3 （STAT3） and a_nti-apoptotic factor （survivin） protein in hepatoma cells was examined by Immunohistochemistry. Results： Q-SNEDDS significantly inhibited the proliferation of hepatoma cells in a concentration-dependent manner. After 24 h of Q-SNEDDS treatment, the total apoptosis rate was 25.8%; STAT3 and survivin protein expression was down-regulated in the Q-SNEDDS group. Conclusion： Q-SNEDDS may play a role in inducing apoptosis by inhibiting the expression of STAT3 and survivin proteins.

关 键 词：Quercetin self-microemulsion Hepatocellular carcinoma APOPTOSIS 

分 类 号：R73[医药卫生—肿瘤]