酚类物质对杉木幼苗^(15)N养分吸收、分配的影响  

EFFECT OF PHENOLICS ON ^(15)N NUTRIENT ABSORPTION AND DISTRIBUTION OF CUNNINGHAMIA LANCEOLATA

作　　者：陈龙池[1] 廖利平[1] 汪思龙[1] 黄志群[1] 高洪[1] 

机构地区：[1]中国科学院沈阳应用生态研究所会同森林生态实验站,沈阳110016

出　　处：《植物生态学报》2002年第5期525-532,共8页Chinese Journal of Plant Ecology

基　　金：中国科学院知识创新工程资助项目 (KZCX2_40 6 )

摘　　要：应用15N同位素示踪技术 ,通过盆栽实验研究了香草醛和对羟基苯甲酸对杉木 (Cunninghamialanceolata)幼苗N素养分吸收、分配的影响 ,结果发现香草醛及其与对羟基苯甲酸的混合物 (浓度比 1∶1)明显抑制了杉木幼苗的生长和对15NO-3 离子的吸收 ,10mmol·L-1的香草醛使杉木幼苗根、茎、叶生物量分别下降了 2 5 .3%、13.5 %、5 .7% ,15N吸收量分别减少了 38.5 %、48.1%、46 .5 % ;10mmol·L-1的混合物使杉木幼苗根、茎、叶生物量分别下降了33.5 %、36 .0 %、2 1.8% ,15N吸收量分别减少了 34 .3%、5 8.4%、49.3%。同时还发现香草醛和对羟基苯甲酸的混合物 (浓度比 1∶1)加重了对杉木幼苗生长的抑制作用 ,这说明两种酚类物质之间存在着协同作用。香草醛和混合物影响了杉木幼苗不同器官中N素养分的分配 ,增加了根系中N素的分配率 ,而减少了茎、叶中N素的分配率。香草醛等酚类物质浓度较高时能够抑制杉木幼苗养分的吸收和地上部分养分的分配 ,导致杉木缺乏生长所必须的养分 。Pure Chinese fir (Cunninghamia lanceolata) plantations, a major commercial tree species in South China, have been extensively replanted on the same site in successive rotations, in response to the growing need for timber. The resultant soil degradation has led to poor establishment and decline in productivity which has become a major barrier to the sustainable management of Chinese fir plantations. Recently, many documented studies indicate that allelopathy within Chinese fir plantations is likely one of the causes of soil degradation. According to some laboratory studies, extracts of Chinese fir roots, fresh leaves, and litter including phenolics such as vanillin, ferulic acids and P-hydroxybenzoic acids, inhibit Chinese fir seed germination. However, no allelopathic responses have been demonstrated. How do the allelochemicals, e.g. phenolics, affect the growth of Chinese fir seedlings? This has not yet been studied. In this study on nutritional aspects of allelopathy in Chinese fir, the effects of different concentrations of vanillin and P-hydroxybenzoic acid on 15NO - 3 ion absorption and distribution in Chinese fir seedlings was examined using the 15N isotope tracer technique. Vanillin, P-hydroxybenzoic acid, and a combined 1∶1 solution of these two materials were diluted to four different levels: 10 mmol·L -1, 1 mmol·L -1, 0.1 mmol·L -1, and 0.01 mmol·L -1. Distilled water was used as the control. One-year-old Chinese fir seedlings were planted in separate pots, fertilized with 200 mg NaNO - 3 enriched 21.97 percent Na 15NO - 3, and grown outdoors in overshadow. Each pot was treated every half month with 100 ml of a particular concentration of vanillin, P-hydroxybenzoic acid solution, or the 1∶1 mixed solution. Each treatment consisted of five replicates of Chinese fir seedlings. After six months height, stem diameter at ground line, total biomass, total N, and 15N content of Chinese fir seedlings were recorded. Results showed that vanillin inhibited both

关 键 词：酚类物质 杉木幼苗 养分吸收 香草醛 对羟基苯甲酸 ^15N同位素 养分分配 化感作用 

分 类 号：Q946[生物学—植物学]