油菜花粉黄酮对氧自由基致鼠红细胞膜损伤的保护作用  

Protection of role Cole Pollen Flavonoids on Oxidative Injury of Erythrocyte Membrance(Induced by Oxygen Radicals)by Florescence Probe

作　　者：曹炜[1] 赵长琦[1] 尉亚辉[1] 

机构地区：[1]西北大学生命科学学院,西安710069

出　　处：《食品科学》2002年第9期115-118,共4页Food Science

基　　金：陕西省自然科学基金(2000SM16);西安市科委科技攻关项目(G9868)

摘　　要：应用1,6-二苯基-1,3,5-已三烯(DPH)为荧光探针,超氧阴离子自由基和羟基自由基致鼠红细胞膜氧化损伤实验模型,研究了油菜花粉黄酮对鼠红细胞膜氧化损伤的影响。结果表明,超氧阴离子自由基和羟基自由基均能引起鼠红细胞膜脂质过氧化反应,脂质过氧化产物丙二醛含量显著升高,膜脂流动性下降。将鼠红细胞膜预先用油菜花粉黄酮处理后,膜脂的MDA含量明显下降,呈现剂量与效应关系,膜脂流动性显著提高,表明油菜花粉黄酮超氧阴离子自由基和羟基自由基引起的鼠红细胞膜的氧化损伤有保护作用。dipheny1-1,3,5-hexatriene,a kind of fluorescence probe,was used to observe the effects of cole pollen flavonoids on the oxidative damage in rat erythrocyte ghost induced by peroxy radical and hydroxy radical.The results demonstrated that both peroxy radical and hrdroxy radical increased the content of malondialdehyde(MDA),decreased membrance fluidity significantly,and caused lipid peroxidation of rat erythrocyte ghost.But cole pollen flavonoids could decrease the content of MDA remarkably,improve the membrance fluidity of rat erythrocyte membrance fluidity and inhibit the lipid peroxidation in rat erythrocyte membrance .These results suggested that cole pollen was a kind of natural antioxidant and could prevent lipid peroxidation in rat erythrocyte membrance damage induced by peroxy radical and hydroxy radical.

关 键 词：油菜花粉黄酮 荧光探针法 红细胞膜损伤 保护作用 

分 类 号：S896.4[农业科学—特种经济动物饲养]