Effects of dl-praeruptorin A on interleukin-6 level and Fas, bax, bcl-2 protein expression in ischemia-reperfusion myocardium  

前胡甲素对缺血再灌注心肌IL-6水平及Fas,bax,bcl-2蛋白表达的影响(英文)

作　　者：常天辉[1] 刘晓阳[1] 章新华[1] 王怀良[1] 

机构地区：[1]中国医科大学临床药理教研室,沈阳中国110001

出　　处：《Acta Pharmacologica Sinica》2002年第9期769-774,共6页中国药理学报（英文版）

基　　金：Project partly supported by the Key Foundation of the Ministry of Public Health of China,№ 88402257; Project partly supported by he Key Foundation of the Ministry of Public Health of China,№ 88402257.

摘　　要：AIM: To investigate the effects of dL-praeruptorin (Pd-Ia) on interleukin-6 (IL-6) level and apoptosis-related protein expression in ischemia-reperfusion myocardium. METHODS: Left anterior descending coronary artery was subjected to 30 min ischemia followed by 120 min reperfusion in open-chest anesthetized rats. Serum IL-6 level was measured by radioimmunoassay. Apoptosis-related protein Fas, bax, and bcl-2 expression was detected by immunohistochemistry and computer image analysis system. Infiltration of neutrophils was observed using Hematoxylin-Eosin staining under optical microscope. RESULTS: Pd-Ia 2.0 mg·kg-1iv lowered serum IL-6 level and Fas, bax, bcl-2 expression under conditions with hypotension and without changes on heart rate, but increased the ratio of bcl-2/bax. There existed a close linearity and positive correlation between IL-6 level and Fas, bax, bcl-2 expression. Whereas, the infiltration of neutrophils was mild. CONCLUSION: Pd-Ia elicits a novel target in the therapeutic prevention of postischemic cardiomyocyte death. The reason might be associated with modulating the expression of some immediate-early genes including IL-6, Fas, bax, and bcl-2 in ischemia-reperfusion myocardium.目的:研究前胡甲素对缺血再灌注心肌IL-6水平及凋亡相关蛋白表达的影响.方法:麻醉开胸大鼠左前降枝冠状动脉蒙受30分钟缺血及120分钟再灌注.放射免疫法测定血清IL-6水平;免疫组化法和计算机图像分析系统检测凋亡相关蛋白Fas,bax及bcl-2的表达;苏木精-依红染色法染色并于光镜下观测嗜中性白细胞的浸润.结果:前胡甲素2·0 mg·kg^(-1) iv,在降压和不影响心率的情况下,减少IL-6水平及Fas,bax,bcl-2蛋白的表达,但增加bcl-2/bax的比率.IL-6水平及Fas,bax,bcl-2蛋白表达之间有密切的线性正相关,而嗜中性白细胞只有轻微浸润.结论:前胡甲素防治缺血后心肌细胞死亡出现新靶位,可能与机体在心肌缺血再灌注期间调控即早基因IL-6,Fas,bax,bcl-2的表达有关.

关 键 词：dl-praeruptorin A MYOCARDIUM ISCHEMIA reperfusion injury apoptosis INTERLEUKIN-6 

分 类 号：R285[医药卫生—中药学]