不同肝病患者血清中肝再生增强因子水平的检测及意义  

Detection of Augmenter of Liver Regeneration in Sera of Patients with Various Liver Diseases

作　　者：周平 杨晓明[1,2] 李奇芬 何浩 贺福初 张木森 

机构地区：[1]军事医学科学院放射医学研究所 [2]第三军医大学西南医院传染科,重庆400036

出　　处：《空军总医院学报》1998年第2期67-69,73,共4页Journal of General Hospital of Air Force,PLA

基　　金：国家863 计划资助项目

摘　　要：目的　建立血清肝再生增强因子（ＡＬＲ）的酶联免疫检测方法，了解不同肝病患者血清中ＡＬＲ 水平及其意义。　方法　利用ＡＬＲ的酶联免疫方法检测不同肝病患者外周血清中ＡＬＲ 水平。　结果　所建立的ＡＬＲ 酶联免疫方法其最低检测到的ＡＬＲ 浓度为０．１ μｇ／Ｌ，在１０ ｎｇ／ｍ ｌ范围内抗体与ＡＬＲ 的反应呈良好的线性关系，与ＴＧＦ－α，ＥＧＦ等其它促肝细胞增殖因子无任何交叉反应。重型肝炎患者血清中 ＡＬＲ 水平最高，急性肝炎次之，两者均非常显著高于正常人，慢性肝炎，肝炎后肝硬变，原发性肝癌和肝外疾病患者（Ｐ＜ ０．０５ 和Ｐ＜ ０．０１）；慢性肝炎和肝炎后肝硬变患者血清中ＡＬＲ 水平亦明显高于正常人（Ｐ＜ ０．０５）；原发性肝癌和肝外疾病患者血清ＡＬＲ 水平与正常人无明显差异（Ｐ＞ ０．０５）。　结论　ＡＬＲ 酶联免疫检测方法对于不同肝病患者血清中ＡＬＲ 的检测具有良好的特异性和敏感性；肝病患者血清 ＡＬＲIn order to develop a method of enzyme linked immunoassay(ELISA) for measurement of serum augmenter of liver regeneration(ALR) and to investigate the levels of ALR in serum of patients with various liver diseases. Method: The serum ALR of patients with various diseases was detected by means of ELISA technique. Results: The lowest concentration to be detected by such a technique was 0.1ng purified ALR/ml. The standard curve for ALR revealed that the latter, within the range of 0.1 10.0ng/ml, was linearly related to the antibody and there was no cross reactivity to TGF α, EGF or other hepatocyte proliferative factors. The serum ALR was the highest in severe hepatitis and the second highest in acute hepatitis. The serum ALR in Both the 2 conditions was markedly higher than that in normal subjects, and that in chronic hepatitis, liver cirrohosis, primary hepatocelullar carcinoma and extra-hepatic diseases ( P<0.05 or P<0.01 ); Serum ALR in chronic hepatitis and liver cirrohosis was significantly higher than that in normal subjects ( P<0.05 ). In patients with primary hepatocellular carcinoma and extra-hepatic diseases, serum ALR was not different from that of normal subjects (P>0.05) . Conclusion: The ELISA technique to assay serum ALR was highly specific and sensitive and the increase of serum ALR was strongly related to liver injury and liver regeneration.

关 键 词：血清 肝再生增强因子 酶连接免疫吸附测定 病毒性肝炎 肝硬变 肝肿瘤 临床意义 

分 类 号：R575.2[医药卫生—消化系统] R512.6[医药卫生—内科学]