实验性糖尿病视网膜微血管病变的病理研究  

Pathological research in experimental diabetes in rat

作　　者：魏玲格[1] 王长龄[1] 段惠军[2] 

机构地区：[1]河北医科大学第二附属医院眼科 [2]河北医科大学病理教研室

出　　处：《中华眼科杂志》2002年第9期523-525,共3页Chinese Journal of Ophthalmology

基　　金：河北省自然科学基金资助项目 (3 9842 6)

摘　　要：目的　观察糖尿病视网膜病变 (diabeticretinopathy,DR)的组织学改变。方法　应用光镜、免疫组织化学、电镜及组织化学电镜等技术 ,研究在不同时间点Spregue Dawley(SD)大鼠视网膜毛细血管基底膜中的Ⅳ型胶原蛋白及层黏连蛋白和视网膜毛细血管基底膜的厚度 ,以及其负电荷位点数目的变化。结果　随着糖尿病病程的发展 ,视网膜毛细血管基底膜不断增厚并伴有Ⅳ型胶原蛋白及层黏连蛋白的增加 ,同时负电荷位点数目减少。结论　视网膜毛细血管基底膜增厚 ,Ⅳ型胶原蛋白及层黏连蛋白的增加 ,负电荷位点数目减少可能是导致DR渗出性病变的病理基础。Objective To study dynamically the pathological changes of diabetic retinopathy (DR). Methods The techniques of light microscopy, immunohistochemical staining, electron microscopy and histochemical electron microscopy were used to quantitatively analyze the Type Ⅳ collagen, laminin and their anionic sites in the rat retinal capillary basement membrane (RBM). Results RBM became gradually thickened and was accompanied with an increased staining reaction for type Ⅳ collagen and laminin and decrease of anionic sites in number. Conclusion The increase in thickness, in type Ⅳ collagen and laminin and the decrease of the anionic sites in the RBM may be the pathological changes of the exudative lesion in DR.

关 键 词：糖尿病视网膜病 动物实验 视网膜血管 病理学 

分 类 号：R774.1[医药卫生—眼科]