碱性成纤维细胞生长因子对鼠视网膜缺血再灌注损伤的治疗作用  

An experimental study of therapeutic effect of basic fibroblast growth factor on experimental retinal ischemia/reperfusion injury

作　　者：牛膺筠[1] 张瑞[1] 周占宇[1] 王红云[1] 刘夫玲[1] 

机构地区：[1]青岛大学医学院附属医院眼科,266003

出　　处：《中华眼科杂志》2002年第9期530-534,共5页Chinese Journal of Ophthalmology

基　　金：山东省教育委员会基金资助项目 (JOOK5 3 )

摘　　要：目的　探讨玻璃体腔注射碱性成纤维细胞生长因子 (basicfibroblastgrowthfactor,bFGF)对实验性视网膜缺血再灌注损伤的治疗作用。方法　采用升高眼内压的方法 ,制作实验性视网膜缺血再灌注损伤大鼠模型。将Wistar大鼠随机分为正常组、缺血组及治疗组。再灌注开始时 ,缺血组大鼠玻璃体腔内注入平衡盐溶液 ,治疗组注入bFGF 2 μg。观察再灌注后不同时间段各组鼠视网膜组织学及超微结构变化 ,光镜下计数视网膜神经节细胞 (retinalganglioncells,RGCs) ,应用图像分析系统测量视网膜内层厚度。结果　视网膜缺血再灌注早期 ,治疗组大鼠视网膜水肿较缺血组轻 ,各时间段治疗组大鼠视网膜内层厚度均较缺血组厚 ,治疗组大鼠RGCs数目多于缺血组。再灌注后 16 8h ,缺血组大鼠神经纤维层厚度及RGCs数目明显低于正常组 ,而治疗组大鼠神经纤维层厚度及RGCs数目与正常组比较 ,差异无显著意义 (P <0 0 5 )。再灌注后 2 4h ,缺血组大鼠RGCs核膜肿胀 ,线粒体嵴模糊不清 ,可见凋亡小体 ,神经纤维中微管模糊、减少甚至消失 ;而治疗组仅部分核膜轻度肿胀 ,胞浆内细胞器丰富 ,线粒体及微管结构较清楚。结论　大鼠玻璃体腔注射bFGF对实验性视网膜缺血再灌注损伤具有治疗作用。Objective To evaluate the therapeutic effect of basic fibroblast growth factor (bFGF) injected into the vitreous cavity on experimental retinal ischemia/reperfusion injury Method The Wistar rat model of experimental retinal ischemia/reperfusion injury was made by increasing the intraocular pressure The rats were divided into normal, ischemia and treatment groups randomly At the beginning of reperfusion, normal saline was injected into the vitreous cavity in ischemia group and 2 μg of bFGF was injected into the treatment group The histological and ultrastructural changes in retina of different time after reperfusion were observed The retinal ganglion cell number was counted by using microscope The thickness of inner layer of retina was measured by using Image Diagnosis System Result In the early period of retinal ischemia/reperfusion injury, the edematous status of retina of treatment group was lighter than that of the ischemia group The retinal inner layer thickness and the RGC number of treatment group were greater than that of the ischemia group during all the post reperfusion stages At 168 hours after reperfusion, the thickness of the retinal nerve fiber layer and the RGC numbers of ischemia groups were obviously lower than that of the normal groups But the differences of thickness of the nerve fiber layer and RGC number between the treatment and normal group were of no statistical importance The nuclear membranes of RGCs were edematous and the mitochondrial cristae were unclear at 24th hour after reperfusion, the apoptotic body can be found, and the microtubules in nerve fibers became unclear and even disappeared in the ischemic group While in the treatment group, the nucleus was clear, only part of the nuclear membrane was edematous, the cell organs were abundant and the structures of mitochondria and microtubules were clear Conclusion Injection of bFGF into the vitreous cavity has the therapeutic effect on experimental retinal ischemia/reperfusion injury

关 键 词：再灌注损伤 视网膜 局部缺血 碱性成纤维细胞生长因子 WISTAR大鼠 治疗 

分 类 号：R774.1[医药卫生—眼科]