体外培养人成纤维细胞转化生长因子-β_1自分泌规律的研究  

AUTOCRINE REGULATION FOR TRANSFORMING GROWTH FACTOR-β_1 BY FIBROBLAST IN VITRO

作　　者：张洁[1] 林苹[1] 黄鲁刚[2] 陆燕蓉[1] 周宏远[1] 黄孝忠 宁其志 

机构地区：[1]四川大学华西临床医学院肿瘤研究所,成都610041 [2]四川大学华西临床医学院小儿外科

出　　处：《中国修复重建外科杂志》2002年第5期319-321,共3页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家自然科学基金重点资助项目 (3980 0 1 4 7)~~

摘　　要：目的　探讨体外培养人成纤维细胞转化生长因子 -β1 (TGF-β1 )的自分泌规律。方法　应用健康人包皮环切术所得包皮 ,通过体外成纤维细胞传代培养共 12代 ,运用 EL ISA法 ,分别检测不同代次成纤维细胞 TGF-β1 浓度 ,并测定第 6代成纤维细胞在不同时间培养上清液中 TGF-β1 的浓度。结果　自第 3代开始 ,成纤维细胞培养上清液可检测到 TGF-β1 ,并逐渐升高 ,至第 6代达分泌高峰 (45 0 ng/ L ) ,第 11、12代不能测到 ;而第 6代成纤维细胞 ,培养上清液中 TGF-β1 含量以第 5天的检测值最高 (6 80 ng/ L ) ,是第 1天检测值 (2 80 ng/ L )的 2 .5倍。结论　成纤维细胞 TGF-β1 自分泌能力 ,是由弱到强再逐渐减弱的过程 。Objective To explore the autocrine regulation for the transforming growth factor β 1 (TGF β 1) by the fibroblasts in vitro. Methods Fibroblasts were cultured in vitro which isolated from the normal prepuce by circumcision. TGF β 1 concentration were determined with ELISA for the different passages and the different time at the same passage. Results The TGF β 1 concentration was different for the distinct passage of fibroblasts and achieved the peak (450 ng/L) at the sixth passage. Among the same fibroblasts, the TGF β 1 concentration was different in the various days and passage of the summit (680ng/L) on the fifth day, which was 2.5 times as high as that of the first day. Conclusion The fibroblasts possess the ability of autocrine of the TGF β 1 and have some regularity. It will provide the theory basis for the research about TGF β 1 regulation mechanism and the medical application about salvia miltiorrhiza.

关 键 词：体外培养 人成纤维细胞 转化生长因子-Β1 自分泌 ELISA法 创伤修复 

分 类 号：R363[医药卫生—病理学]