噁喹酸、氟甲喹在鸡组织中残留量的检测方法研究  

Study on the Detection Method of Oxolinic Acid and Flumequine Residues in Chicken Tissues

作　　者：仲锋[1] 汪霞[1] 董琳琳[1] 彭莉[2] 崔庶 岳秀英[2] 

机构地区：[1]中国兽医药品监察所,北京100081 [2]四川省兽药监察所,四川成都610041

出　　处：《中国兽药杂志》2002年第9期19-21,共3页Chinese Journal of Veterinary Drug

摘　　要：用荧光检测的高效液相色谱法同时检测鸡组织中E喹酸、氟甲喹的残留量。用1mol/L磷酸二氢钾溶液作为提取液，过C18固相萃取柱净化、浓缩。HPLC条件：荧光检测器，激发波长325nm；发射波长369nm。流动相：0.02mol/L磷酸－乙腈－四氢呋喃（69：16：15）。E喹酸、氟甲喹标准曲线线性范围在10－500ng/mL。对不同组织的3种浓度进行回收率测定，其回收率在61％－92％之间，变异系数在15％以内。E喹酸、氟甲喹的检测灵敏度为20ng/g。The method of high-performance liquid chromatography (HPLC) with fluorescence detection wasused for the simultaneous determination of oxolinic acid and flumequine residues in chicken tissues. The drugswere extracted with 0.1 mol/L aqueous potassium dihydrogenphosphate, purified and concentrated by passingthrough Bond Elut C18 solid-phase extraction cartridges. The HPLC separation was carried out on a Luna C18 5μmcolumn (150 mm×4.6 mm i.d.) with 0.02 mol/L phosphoric acid - acetonitrile - tetrahydrofuran (69∶16∶15)as the mobile phase. A fluorescence detector was used at an excitation wavelength of 325 nm and an emissionwavelength of 369 nm. The calibration graphs were linear between 10 ng/mL and 500 ng/mL for oxolinic acid andflumequine. Average recoveries of the drugs from chicken tissues fortified at a level of 20~300 ng/g were 61%~92%.Coeffcients of variation were 15%, and the detection limits for oxolinic acid and flumequine were 20 ng/g.

关 键 词：E喹酸 氟甲喹 鸡 组织 残留量 高效液相色谱 检测技术 

分 类 号：S858.31[农业科学—临床兽医学] S859.84[农业科学—兽医学]