放射自显影示踪研究^(153)Sm-EDTMP进入骨肉瘤细胞的动态  

The Entrance to Bone Tumor Cells of ^(153)Sm-EDTMP by Microautoradiographic Tracing

作　　者：朱寿彭[1] 肖东[1] 吴越[1] 

机构地区：[1]苏州大学放射毒理室,江苏苏州215007

出　　处：《同位素》2002年第3期148-150,共3页Journal of Isotopes

基　　金：国家自然科学基金资助项目(39470389)

摘　　要：采用微观放射自显影示踪法探讨了153Sm-EDTMP在不同照射时间间隔进入骨肉瘤细胞的过程,及其在瘤细胞中的滞留动态。结果表明:153Sm-EDTMP首先向骨肉瘤细胞呈亲膜性积聚,随后透过骨肉瘤细胞膜,进入瘤细胞内;也可被瘤细胞吞噬进入细胞内,以吞噬小体形式沉积;在呈现凋亡形态的骨肉瘤细胞中,153Sm-EDTMP可在膜包裹着的凋亡小体中呈现。因此,153Sm-EDTMP内照射进入骨肉瘤时所诱发的瘤细胞凋亡,是153Sm-EDTMP能透入瘤细胞膜,以及被瘤细胞吞噬进入瘤细胞中沉积所致。The dynamic process of 153SmEDTMP entering into bone tumor cells and its behavior characteristic in osteosarcoma cells are studied by microautoradiographic tracing. The results show that 153SmEDTMP condense on membrane of osteosarcoma cells at first, then infiltrate through cell membrane or phagocytized by osteosarcoma cells and distributed in the form of phagosomes. In apoptotic osteosarcoma cells 153SmEDTMP could be observed in the membranebounded apoptotic bodies. So, the progression of apoptosis in osteosarcoma cells induced by 153SmEDTMP is dependent on the infiltration and phagocytosis of 153SmEDTMP through cell membrane.

关 键 词：放射自显影 ^153SM-EDTMP 骨肉瘤细胞 示踪法 标记 钐153 放射性核素 治疗 

分 类 号：R738.1[医药卫生—肿瘤] R730.55[医药卫生—临床医学]