硝酸还原酶在巯基自组装膜上的电化学性质  

Electrochemical Properties of Nitrate Immobilized on Gold Electrodes with 2-aminoethanethiol SAMs

作　　者：宋雅茹[1] 吴红[1] 邵会波[2] 

机构地区：[1]北京师范大学化学系,北京100875 [2]首都师范大学化学系,北京100037

出　　处：《电化学》2002年第3期333-336,共4页Journal of Electrochemistry

基　　金：国家自然科学基金 (2 99730 2 6 );北京市自然科学基金 (2 992 0 0 7);教育部高等学校骨干教师资助计划资助

摘　　要：应用自组装技术将硝酸还原酶 (NR)构筑在硫醇自组装单分子膜上 ,研究了Au/半胱胺 /NR和Au/半胱胺Nitrate reductase (NR) and phospholipids were immobilized on gold electrodes with 2_aminoethanethio(AET) SAMs. After Au/AET/NR and Au/AET/NR/lipid were soaked in pH 7 phosphate buffers for days,a pair of pre_reversible peak appeared at around 0.202 V and 0.149 V,respsctively, irregularly grew with soaking time in blank buffer.The cathodic peak current(i pcl )of Au/AET/NR system had linear relationship with scan rate between 0.02 and 0.5 V.s -1 ,while i pcl was proportional to V 1/2 in Au/AET/NR/lipid system.The direct electron transfer of enzyme electrodes was strongly affected by pH.Compared with Au/AET,NR exhibited a higher electrochemical activity in Au/AET/lipid system.

关 键 词：电化学性质 硝酸还原酶 卵磷脂 半脘胺自组装膜 硫醇 

分 类 号：O646[理学—物理化学]