端粒酶基因hTR在胃癌中的表达及临床意义  

Expression of telomerase gene hTR and clinical association in gastric cancer

作　　者：李贵新[1] 马世庆[2] 徐功立[1] 

机构地区：[1]山东大学临床医学院山东省立医院肿瘤研究治疗中心,山东济南250021 [2]山东省潍坊市人民医院外科,山东潍坊261041

出　　处：《中国现代普通外科进展》2002年第3期162-164,共3页Chinese Journal of Current Advances in General Surgery

基　　金：山东省科学技术攻关计划基金资助项目 (98115 45 0 3B)

摘　　要：目的 :探讨胃癌中端粒酶hTR基因表达与临床参数的关系 ,评价hTR表达在胃癌诊断中的价值。方法 :用原位杂交的方法检测端粒酶hTR基因在胃癌、癌旁组织和胃良性病变中的表达和分布情况 ,并分析与各临床参数的关系。结果 :5 7例胃癌中hTR基因表达阳性率为 94 .7% (5 4 / 5 7) ,5 7例癌旁组织中hTR基因表达阳性率为 0 (0 / 5 7) ,6 9例胃良性病变中hTR基因表达阳性率为 2 .9% (2 / 6 9)。胃癌组织中hTR基因的表达与癌旁组织、胃良性病变比较差异有显著性 (P均 <0 .0 0 1) ,并与肿瘤分化程度、淋巴结转移相关 (P均 <0 .0 5 ) ,与肿瘤分期无相关性 (P >0 .0 5 )。结论 :端粒酶hTR基因表达检测在胃癌诊断中可能有一定的价值。Objective:To investigate the correlation between the expressions of telomerase gene hTR (human telomerase reverse transcriptase)and clinical parameters in gastric cancer,and to evaluate the role of detection of hTR gene in gastric cancer diagnosis.Methods:With in situ hybridization,the expression and distribution of telomerase gene hTR was observed in 57 cases of gastric cancer tissues,57 cases of tumor adjacent tissues,69 cases of benigh lesions.The results were statistically analyzed.Results:hTR was detected in 94.7%(54/57) of gastric cancer.While in the adjacent tissues,the positive rate was 0(0/57).The positive rate for hTR was 2.9%(2/69) in benign lesions.The positive detection of hTR expression in cancer group significantly different from those in the adjacent tissues and benigh lesions group(P both<0.001).The expression of hTR correlated well with degrees of differientiation and lymph node metastasis(P both<0.05),but not with tumor stage(P>0.05).Conclusion:The detection of telomerase hTR gene expression may be useful in diagnosis in gastric cancer.

关 键 词：端粒酶 基因 HTR 胃癌 临床意义 基因表达 

分 类 号：R735.2[医药卫生—肿瘤] Q786[医药卫生—临床医学]