灰树花不同菌株液体深层培养蛋白质含量的测定  

The Determination of Protein Content of DifferentGrifola frondosa Strains Under Submerged Culture

作　　者：宋爱荣[1] 张玉娜[1] 孙克梅[1] 张英昊[1] 万平平[1] 

机构地区：[1]莱阳农学院植保系,山东莱阳265200

出　　处：《食用菌学报》2002年第3期9-12,共4页Acta Edulis Fungi

摘　　要：在液体培养条件下测定了发酵液中 5个灰树花菌株蛋白质含量的变化。结果表明 ,接种后第 6天发酵液中蛋白质含量最高 ,Gf96 1菌株发酵液中的蛋白质含量为 0 .5 8% ,Gf96 2菌株发酵液中的蛋白质含量最低 ,为 0 .30 %。随着培养时间的延长 ,发酵液中蛋白质含量逐渐下降。接种后第 9天最低 ,随后又呈上升趋势 ,但上升的幅度较小。The variation of protein content of 5  Grifola frondosa strains under submerged culture conditions was determined in this study. The result showed that the protein content of the fermented broth was the highest at the sixth day of spawning, and that the protein content of Gf961 in the fermented broth was the highest(0.58%), while that of Gf962 was the lowest(0.30%). It was also confirmed in our experiments that the protein content in the fermented broth reduced gradually along with the extending of the culture time, and reached the lowest at the 9th day of spawning, but it increased again later with small rate of increment. So the experiment result offered some theoretical bases for the determination of optimum time to extract proteins from the fermented broth.

关 键 词：灰树花 菌株 液体深层培养 蛋白质含量 测定 

分 类 号：S646[农业科学—蔬菜学]