细胞毒药物(Ge mcitabine)治疗人胰腺癌裸鼠胰腺原位移植癌的实验研究  

An experimental study on therapeutic effects of Gemcitabine on human pancreatic carcinoma orthotopically implanted in nude mice

作　　者：贾林[1] 袁世珍[2] 黄文革[3] 郭芬芬[3] 

机构地区：[1]广州市第一人民医院消化内科,广州市510180 [2]中山医科大学孙逸仙纪念医院消化内科 [3]中山医科大学动物实验中心

出　　处：《中华肝胆外科杂志》2002年第9期557-559,共3页Chinese Journal of Hepatobiliary Surgery

摘　　要：目的　探讨细胞毒药物 (Gemcitabine)对胰腺癌裸鼠原位移植模型 (SOI)生长、转移的抑制作用。方法　SOI模型分为Ⅰ组 (Gemcitabine 10 0mg/kg)、Ⅱ组 (Gemcitabine 5 0mg/kg)和对照组。Gemcitabine于术后第 0 ,3,6 ,9天给药 ,ip ,术后第 10周处死裸鼠 ;并对肿瘤组织的增殖指数 (PI)、凋亡指数 (AI)以及PI/AI进行分析。结果　 (1)Ⅰ组能显著抑制胰腺癌生长 ,Ⅱ组则无显著的肿瘤抑制作用 ,但两者对胰腺癌转移和预后均无显著疗效 ;(2 )Ⅰ组可显著降低肿瘤增殖指数 (PI) ,增大凋亡指数 (AI) ,PI/AI显著降低。结论　Gemcitabine单用能有效抑制胰腺癌生长 。Objective To investigate the anti tumor and anti metastasis effects of Gemcitabine on the model of human pancreatic carcinoma established by surgical orthotopic implantation (SOI) in nude mice. Methods The SOI model in nude mice were divided into control group, G100 group and G50 group receiving normal saline, 100 mg/kg Gemcitabine and 50 mg/kg Gecitabine i.p. on days 0, 3, 6 and 9 after the transplantation, respectively. The mice were killed at the 10th week after the transplantation. Then the apoptosis index (AI), the PCNA index (PI) and ratio of PI/AI were determined. Results (1) The inhibitory effects of Gemcitabine on the tumor was more significant in G100 group than in G50 group. No significant improvement in tumor metastasis and survival rate of nude mice was seen in G100 group and G50 group. (2) In G100 group, the level of PI and the ratio of PI/AI were markedly reduced while the level of apoptosis of pancreatic carcinoma cells (AI) remarkably increased. Conclusions Gemcitabine used alone can significantly inhibit growth of pancreatic carcinoma but can not markedly reduce its metastasis.

关 键 词：胰腺肿瘤 肿瘤生长 肿瘤转移 2' 2’-二氟脱氧胞苷 药理学 细胞毒药物 胰腺癌 胰腺原位 移植癌 

分 类 号：R735.9[医药卫生—肿瘤] R965[医药卫生—临床医学]