紫杉醇诱导成骨肉瘤细胞凋亡  

PACLITAXELINDUCED APOPTOSIS IN HUMAN OSTEOSARCOMA CELL LINE

作　　者：丁勇[1] 范清宇[1] 张殿忠[1] 殷剑宁[1] 

机构地区：[1]第四军医大学唐都医院全军骨肿瘤研究所,西安710038

出　　处：《中国现代医学杂志》2002年第13期1-3,共3页China Journal of Modern Medicine

基　　金：第四军医大学博士基金资助课题 (1998-13 6)

摘　　要：目的 :探讨紫杉醇对成骨肉瘤细胞的杀伤作用及机制。方法 :应用形态学观察 ,MTT分析法 ,DNA梯状电泳 ,流式细胞术检测术 ,研究紫杉醇对成骨肉瘤细胞系SOSP - 96 0 7的细胞毒效应。结果 :紫杉醇作用后成骨肉瘤细胞形态改变明显 ,存活率明显下降。形态学观察 ,DNA梯状电泳 ,流式细胞术检测术证实紫杉醇可诱导成骨肉瘤凋亡。结论 :紫杉醇在体外对成骨肉瘤细胞株SOSP - 96 0 7有较强的杀伤作用 。Objective:To probe the cytotoxic effect of paclitaxel on human osteosarcoma cell line SOSP-9607 and its mechanism.Methods:The cytotoxic effect of paclitaxel on human osteosarcoma cell line SOSP-9607 was examined by MTT assay, morphological observation, gel electrophoresis of DNA, and cell morphology.Results:Distinct morphological change and obvious decreased survival rate was observed after the action of paclitaxel. Morphologicalobservation, gel electrophoresis of DNA, and cell morphology also conflrmed the effect of inducing apoptosis of paelitaxel on osteosarcoma cell. Conclusion: The cytotoxic effect of paelitaxel on SOSP-9607 cell is achieved by inducing apoptosis. Also, the killing effect of paclitaxel toward SOSP-9607 cell line was confirmed in vitro.

关 键 词：紫杉醇 成骨肉瘤细胞 细胞凋亡 骨肉瘤 细胞毒性作用 药理学 

分 类 号：R738.1[医药卫生—肿瘤] R979.1[医药卫生—临床医学]