一种Ⅲ型前胶原放射免疫分析方法的建立  

The Establishment of a Radioimmunoassay by Using Type Ⅲ Precollagen

作　　者：柴明胜[1] 陈铁河[1] 彭武林[1] 

机构地区：[1]海军医学研究所,上海200433

出　　处：《海军医学杂志》2002年第3期256-258,共3页Journal of Navy Medicine

摘　　要：目的 :从山羊胎皮中纯化Ⅲ型前胶原 (gPCⅢ ) ,建立血清PCⅢ放免测定法 ,并与用人胎皮提取的Ⅲ型前胶原(hPCⅢ )建立的方法进行比较。方法 :采用分级盐析沉淀和DEAE层析纯化制备 gPCⅢ纯品 ,采用氯胺T法标记纯化的 gPCⅢ ,用完全福氏佐剂在新西兰大白兔皮下注射取得抗gPCⅢ兔血清 ,用双抗体平衡加样法建立放射免疫测定法。并用本法和用hPCⅢ建立的放免法分别测定正常人 10 0例和各型肝病患者 2 0 0例。结果 :两法测定结果完全吻合 ,都显示出肝纤维化组PCⅢ升高最显著 ,慢性肝炎组也有显著升高 ,慢性迁延性肝炎组与其它肝病组无明显升高。结论 :gPCⅢ与hPCⅢ具有斜率近似的曲线 ,两者的基本结构也完全相似。两法测定的临床数据表明 。Objective: To establish a radioimmunoassay (RIA) by using type Ⅲ precollagen (gPCⅢ) isolated and purified from fetal goat skin. Methods: The fetal goat skin precollagens were separated and purified by precipitation goat skin extract with different concentration of salt and DEAE ion changed chromatograph, and labeled with 125 I using a modification of the chloramine T method. Antisera against this purified gPCⅢ were raised in New Zealand white rabbits by multiple interdermal injection of gPCⅢ in saline emulsified with complete Freund's adjuvant; RIA method for gPCⅢ were established using a standard double antibody technique. Serum samples taken from 100 volunteers and 200 patients with suspected liver disease were assayed for PCⅢ using this RIA method and another RIA method developed with the fetal human skin seperately. Results: By both determing methods, the serum PCⅢ was elevated significantly in cirrhotic liver and chronic hepatitis, and was not significantly elevated in patients with chronic persistant hepatitis(CPH) and other liver diseases. Conclusion: The established RIA method for human serum PCⅢ showed a good correlation with the method developed with the fetal human skin in diagnosing liver diseases.

关 键 词：放射免疫分析法 Ⅲ型前胶原 肝硬化 

分 类 号：R575.2[医药卫生—消化系统] R446.61[医药卫生—内科学]