具有肺转移特性的人骨肉瘤细胞系PL-1的建立及其生物学特性观察  

THE ESTABLISHMENT OF AN OSTEOSARCOMA CELL LINE PL-1 AND ITS BIOLOGIC CHARACTERS

作　　者：彭磊[1] 王臻[1] 胡蕴玉[1] 曹云新[1] 张志培[1] 杜俊杰[1] 何锋[1] 常琪[1] 白俊[1] 刘刚[1] 

机构地区：[1]第四军医大学西京医院,西安710032

出　　处：《解放军医学杂志》2002年第9期805-807,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金资助课题 (编号 39770 749)

摘　　要：建立一株人源性骨肉瘤细胞系并进行生物学测定 ,为骨肉瘤研究提供良好的实验模型。无菌切取骨肉瘤手术标本 ,于裸鼠表面包埋接种成功后移植瘤标本体外培养得到细胞株 ,应用形态学观察、流式细胞仪、生长曲线测定、肿瘤标志物测定等方法进行生物学特性研究。结果建立了一株具有肺转移特性的人源性骨肉瘤细胞系PL 1,其成瘤率为 10 0 % ,潜伏期为 12天 ,肺转移率为 10 0 % ;取其 32代细胞进行生物学特性测定 ,发现其形态结构、排列方式、分化程度与原发瘤保持一致 ,染色体形态为人类核型 ,众数在 6 4～ 6 6之间 ,流式细胞仪检测G1期细胞为 5 2 1% ,碱性磷酸酶及骨形成蛋白抗体免疫组化染色为阳性。说明应用裸鼠体内移植建立的人骨肉瘤细胞系PL 1与原发瘤保持相同的生物学特性 ,为一个稳定的细胞系。To establish a xenografted tumor strain of human osteosarcoma in nude mice and a homologous cell line, the osteosarcoma speciments from patients were inoculated into the subcutaneous tissue of nude mice. After the transplanted tumor had grown to about 2cm in diameter, the tumor tissue was cultured in vitro and the continuously growing cells were analyzed by morphology, histochemistry, immunohistochemical straining, cell cycling analysis ,chromosome analysis and heterotransplantation. A newly established cell line designated PL 1 was thus obtained in this laboratory in continuous culture for over 100 passages. Under light and electron microscopes, the cells assumed the main characters of malignancy.Morphological observation, histochemistry analysis and BMP immunohisto chemical straining showed that they had the features of osteosarcoma.The cell cycle analysis showed 53 2% of the cells were in G 1. This cell line could produce ALP and BMP.Chromosome analysis confirmed that they had retained a karyotype of human cancer cells and a hypotriploid feature with a mode number of around 64～66.The tumor formation rate after heterotransplantation was 100%. It had lung metastasis characters. Therefore, PL 1 cell line derived from the xenograft in nude mice has been established and maintained for over 9 months trough 100 passages, and this cell line can provide material and model for further investigation of human osteosarcoma.

关 键 词：骨肉瘤 肿瘤细胞 细胞培养 生物学特性 

分 类 号：R738.102[医药卫生—肿瘤]