地高辛标记探针原位杂交法检测肝病患者外周血单个核细胞中TTV-DNA  

Detection of TTV-DNA in PBMC Using Digoxigenin Labelled Probe by in Situ Hybridization

作　　者：刘暘[1] 其其格[1] 

机构地区：[1]内蒙古医学院第一附属医院传染病教研室,内蒙古呼和浩特010050

出　　处：《标记免疫分析与临床》2002年第3期135-137,共3页Labeled Immunoassays and Clinical Medicine

摘　　要：为检测肝病患者外周血单个核细胞 (PBMC)中TTV -DNA(输血传播病毒脱氧核糖核酸 ) ,以TTVORF1为模板 ,应用地高辛 (Dig)作为标记物 ,经聚合酶链反应 (PCR)制备探针 ,建立原位杂交方法进行检测。结果显示 :血清TTV -DNA阳性组 ,双链探针检测PBMC中TTV -DNA ,阳性检出率为 5 8.0 6 % (18/ 31) ;血清TTV-DNA阴性组 ,双链探针检测PBMC中TTV -DNA ,阳性检出率为 2 7.5 9% (8/ 2 9)。双链探针阳性者 ,再以负链探针检测其复制情况 ,阳性率为 2 2 .2 % (4/ 18)。结论To determine TTV-DNA in PBMC in patients with viral hepatitis, a study of in situ hybridization using digoxigenin labelled probe by PCR method to the TTV ORF1 region was performed on PBMC. Resullts showed that the detection rate of TTV-DNA using double-stranded probe in TTV-DNA positive group in sera was 58.06(18/31), and the detection rate of TTV-DNA using double-stranded probe in TTV-DNA negative group in sera was 27.59(8/29).For TTV-DNA positive group detected by double-stranded probe, then we use negative-stranded probe to detect their replication. The detection rate was 22.2%(4/18). Conclusions: TTV can infect PBMC and replicate in PBMC.

关 键 词：TT病毒 原位杂交 聚合酶链反应 

分 类 号：R575[医药卫生—消化系统]