人参皂甙Rg3对裸鼠原位种植人乳腺浸润性导管癌的抗癌作用及其机制的研究  

Effect of Ginsenoside Rg3 on the Progression of Orthotopically Xenotransplanted Human Breast Cancer in Nude Mice and Its Mechanism

作　　者：陈大富 赵扬冰[2] 白绍槐[3] 史宗道[4] 张杰[5] 

机构地区：[1]广州市海军医院普外科 [2]四川大学华西医院普外科,四川成都610041 [3]四川大学实验动物中心 [4]四川大学华西医学中心临床流行病学教研室 [5]四川大学华西医院病理科

出　　处：《华西医学》2002年第3期296-298,共3页West China Medical Journal

摘　　要：目的 :探讨人参皂甙Rg3(Rg3)抑制裸鼠原位种植人乳腺浸润性导管癌的作用及其作用机制。方法 :采用移植人乳浸润性导管癌的雌性裸鼠 15只 ,随机分成 3组 :Rg3组 ,环磷酰胺 (CTX)组和对照组 ,分别用Rg3、CTX及对照剂连续灌胃 5 6天 ,处死后病理标本用光镜及免疫组化检查。结果 :Rg3组平均瘤重低于对照组(P =0 0 5 ) ,CTX组平均瘤重明显低于对照组 (P <0 0 5 )。免疫组化显示 ,Rg3组的微血管密度 (MVD)及血管内皮生长因子 (VEGF)明显低于对照组 (P <0 0 5 )。结论Objective:To investigate the inhibiting ability of ginsenoside Rg3 on the growth and angiogenesis of orthotopically xenotransplanted human breast infiltrating ductal carcinoma in nude mice.Methods:A total of 15 female nude mice having received xenotransplanted human breast infiltrating ductal carcinoma were randomly divided into 3 groups.Ginsenoside Rg3(5mg/kg,qd),cyclophosphamid(26mg/kg,qod)and control solution(0.5% sodium carboxymethyl cellulose,qd)were given to each group by gastrogavage in 0.5ml volume for 56 days.Breast cancer mass was collected for light microscope observation.The intra-tumoral microvessel density(MVD)and the expression of vascular endothelial growth factors(VEGF)were examined by immunohisto-chemical staining.Results:The tumor weight of CTX group was significantly lower than that of the control group(P<0.05).and the comparision between those of Rg3 group and the control group was nearly aporoaching significant level.The MVD and VEGF of Rg3 group by immunohisto-chemical staining were significantly lower than those of the control group(P<0.05).Conclusions:Rg3 could inhibit the growth and angiogenesis of xenotransplanted human breast infiltrating ductal carcinoma in nude mice.

关 键 词：人参皂甙RG3 人乳浸润性导管癌 微力管密度 血管内皮生长因子 新生血管 

分 类 号：R737.9[医药卫生—肿瘤]