幽门螺杆菌感染BALB/c小鼠模型的研究  

Research of a BALB/c mouse infection model with Helicobacter pylori

作　　者：严英[1] 钱利生[1] 王文风[1] 李雪萍[1] 

机构地区：[1]复旦大学医学院微生物学教研室,上海200032

出　　处：《中国人兽共患病杂志》2002年第5期9-11,F004,共4页Chinese Journal of Zoonoses

摘　　要：目的　建立稳定的Hp感染BALB/c小鼠模型 ,用于Hp致病、免疫和防治的研究。 方法　选用临床分离株 ,应用外科手术法向小鼠胃内直接接种Hp菌液 0 2ml(10 9CFU/ml) ;接种后不同时间取小鼠胃粘膜组织进行细菌学和病理学检查。结果　接种后 2～ 8周 ,小鼠胃内均可分离到Hp ,Hp分离阳性率为 95 %。病理学检查显示鼠胃粘膜明显炎症。感染鼠用阿莫西林治疗后 ,鼠胃组织Hp培养均转阴性。结论　使用Hp鼠胃接种法 。Aim To study the pathogenicity,immune,prevention and cure of H pylori infection,we need to establish a stable infection model of BALB/c mouse with H pylori Methods The BALB/c mouse was inoculated with 0 2ml fresh clinical isolates of H pylori(10 9cFU/ml)to stomach by surgical method Sometimes after inoculation,the mouse stomach was taken out for the examination of bacteriology and pathology Results H pylori were isolated from the stomach of all mice after 2-8 weeks,the positive rate of H pylori isolation was 95% The tissue sections showed obvious gastric inflammation After treatment of those infected mouse with Amoxicillin,isolations of H pylori become negative in the stomach tissues of all mice Conclusion The infection model of BALB/c mouse has been established stably when H pylori was inoculated into the stomach of mouse

关 键 词：幽门螺杆菌感染 阿莫西林 动物模型 细菌学 病理学 胃内直接接种法 

分 类 号：R－332[医药卫生]