多环芳烃和联苯等对鲫鱼肝脏微粒体芳烃羟化酶活性的影响  

Effects of PAHs and Biphenyl-like Compoundson Hepatic Microsome AHH Activitiesof Cyprinus Carpio

作　　者：张民[1] 王君[1] 尹大强[1] 

机构地区：[1]南京大学环境学院,南京210093

出　　处：《上海环境科学》2003年第S2期39-43,192,共6页Shanghai Environmental Sciences

基　　金：国家十五"863"资助项目;编号 2001AA 640601-4

摘　　要：以鲫鱼肝脏微粒体为实验体系,运用体外方法,研究了6种多环芳烃(PAHs)和5种联苯类物质对芳烃羟化酶的活性影响。结果表明,在试验浓度范围内,4-碘-4~′-羟基联苯和4-氰基-4~′-丁基联苯对芳烃羟化酶的活性没有明显的诱导或抑制效应,其余9种化合物对芳烃羟化酶活性都表现出不同程度的诱导,并具有相似的变化趋势。通过对结构相似化合物的诱导能力进行比较,发现并四苯的诱导能力强于3,4-苯基丁苯,二苯并[a,c]蒽的诱导能力强于二苯并[a,h]蒽,4,4~′-二溴联苯的诱导能力要强于联苯,1-甲基菲与3-甲基菲的诱导能力相近。研究揭示,芳烃羟化酶的活性是一个敏感的生物指标,其活性大小与化合物结构有一定的相关性。The aryl hydrocarbon hydroxylase (AHH) activities of Cyprinus Carpio liver microsome induced by six PAHs and five biphenyl-like compounds have been examined in Vitro. It was found that all the tested chemicals induced AHH activity to different extent except 4'-Iodo-biphenyl-4-ol and 4'-Bu-tyl-biphenyl-4-carbonitrile, and the trend of AHH activity changing was similar. The intense of AHH activity induced by chemicals with resemble structure as following order: naphthacene>3H-benzo [de] naphthacene; dibenzo [a, c] anthracene>dibenzo [a, h] anthracene; 4,4'-dibromo-biphenyl> biphenyl; 1-methylphenabthrene≈ 3-methylphenabthrene. Consequently, it was found that AHH activity was a sensitive biomarker and it has certain relationship with chemical structures.

关 键 词：多环芳烃 联苯 肝脏微粒体 芳烃羟化酶 活性 诱导 鲫鱼 

分 类 号：X174[环境科学与工程—环境科学]