多发性硬化患者体内髓磷脂反应性T细胞的横断面和纵向研究  

Cross-sectional and longitudinal analysis of myelinreactive T cells in patients with multiple sclerosis

作　　者：Lünemann J.D. Rückert S. Kern F. F. Zipp 江山 

机构地区：[1]Institute of Neuroimmunology, Neuroscience Research Center, Chari t'e, 10098 Berlin, Germany Dr.

出　　处：《世界核心医学期刊文摘（神经病学分册）》2005年第2期28-29,共2页Digest of the World Core Medical Journals:Clinical Neurology

摘　　要：Activated myelin specific T cells are thought to mediate inflammatory tissue damage in multiple sclerosis (MS). Applying a large panel of myelin antigens, we demonstrate the direct ex vivo detection of viable IFN γ/TNF αproducing CD4 +/CD69+T cells 6 hours after antigenic challenge, by intracellular flow cytome try in 3/33 MS patients and 2/26 healthy controls with calculated frequencies of (mean ±.SEM): 0.031%±0.002%versus 0.037%±0.029%. By comparison, the rece ntly developed IL 7 modified proliferation assay revealed i) a higher number of individuals showing myelin reactivity (17/37 MS patients and 12/24 healthy indi viduals) and ii) a significant difference in the response to myelin basic protei n (MBP) between the two groups in a longitudinal analysis, indicating a higher a ctivity of myelin specific T cells in MS patients. Our data provide new perspec tives in detecting pathogenetically relevant T cells, but clearly demonstrate th e different conclusions which must be drawn from various approaches concerning t he quantification of autoreactive T cells.Activated myelin specific T cells are thought to mediate inflammatory tissue damage in multiple sclerosis (MS). Applying a large panel of myelin antigens, we demonstrate the direct ex vivo detection of viable IFN γ/TNF αproducing CD4 +/CD69+T cells 6 hours after antigenic challenge, by intracellular flow cytome try in 3/33 MS patients and 2/26 healthy controls with calculated frequencies of (mean ±.SEM): 0.031%±0.002%versus 0.037%±0.029%. By comparison, the rece ntly developed IL 7 modified proliferation assay revealed i) a higher number of individuals showing myelin reactivity (17/37 MS patients and 12/24 healthy indi viduals) and ii) a significant difference in the response to myelin basic protei n (MBP) between the two groups in a longitudinal analysis, indicating a higher a ctivity of myelin specific T cells in MS patients. Our data provide new perspec tives in detecting pathogenetically relevant T cells, but clearly demonstrate th e different conclusions which must be drawn from various approaches concerning t he quantification of autoreactive T cells.

关 键 词：多发性硬化 T细胞 髓磷脂 纵向研究 炎性组织 免疫反应 流式细胞仪 特异性 发病机制 定量测定 

分 类 号：R744.51[医药卫生—神经病学与精神病学]