慢性胰腺炎、胰腺癌组织中血管生成与ras基因产物p21蛋白表达的关系及意义  

Relationship between p21^(ras) and angiogenesis in chronic pancreatitis,pancreatic cancer and its clinical significance

作　　者：李兆申[1] 潘雪[1] 任钥欣[1] 许国铭[1] 刘枫[1] 倪灿荣[1] 富克远[1] 

机构地区：[1]第二军医大学附属长海医院消化科,上海200433

出　　处：《胰腺病学》2001年第1期8-10,共3页Chinese JOurnal of Pancreatology

摘　　要：目的 探讨慢性胰腺炎（chronic pancreatitis,CP）、胰腺癌（pancreatic adenocarcinoma,PAC）组织中血管生成与ras基因产物p21蛋白表达的关系及在慢性胰腺炎、胰腺癌演化过程中所起的重要作用。方法 应用免疫组化Envision方法检测7例正常胰腺、24例慢性胰腺炎、24例胰腺癌组织中CD34、p21ras的表达。对CD34阳性血管进行微血管密度（microvessel density,MVD）计数。结果CD34抗体染色大部分毛细微血管和单个内皮细胞。最高微血管染色区域（hotspot）几乎总是在肿瘤与周围组织交界的浸润缘和慢性炎症组织,特别是纤维化组织。对照组正常胰腺组织除腺泡的微血管血供外,叶间结缔组织很少能观察到新生血管。p21ras阳性物可见于增生性胰腺导管上皮细胞及导管腺癌细胞,正常胰腺无表达,癌组织（47.2±9.4）和慢性胰腺炎组织（40.8±7.93）中微血管密度明显高于对照组（9.85±2.86）（P<0.01）。24例胰腺癌组织中p21ras阳性占15例,24例胰腺炎组织中p21ras阳性占11例,两者无显著差异,（x2=1.34,P>0.05）。p21ras阳性胰腺癌组织MVD（52.1±8.3）明显高于p21ras阴性的胰腺癌（39.1±4.1）及慢性胰腺炎组织（35.99±3.36）（P<0.01）,p21ras阳性的慢性胰腺炎组织MVD亦高于p21ras阴性的慢性胰腺炎组织（P<0.01）。Objective To investigate the relationship between the expression of p21ras and angio-genesis in chronic pancreatitis (PC) and pancreatic adenocarcinoma (PAC) and find the role of angiogen-esis and K-ras mutations in the pathogenesis of PAC. Methods In tissue specimens from 24 patients with CP,24 with PAC and 7 controls, microvessel density (MVD) were assessed by immunostaining with CD34. Furthermore the expression of p21ras was observed in all specimens. Results Both CP and PAC exhibited areas of high vascular density(hotspots). Enhanced expression of p21ras in CP and PAC was seen in ductal cells and cancer cells. No p21ras expression was found in control group. The mean microvessel density (MVD) in PAC was 47.2±9.4, in CP 40.8±7.93, and in control group 9.85±2.86 (P<0.01). p21ras were detected in 15/24 PAC and in 11/24 CP, but no statistic different significance was found. The MVD (52.1±8.3)in PAC samples with positive p21ras expression, compared with PAC (39.1±4.1) and CP (35.99±3.36)samples without p21ras expression, was significantly elevated (P< 0.01). Compared to CP samples with no p21ras expression, MVD in CP samples with positive p21ras expression was also significantly elevated (P<0.01). Conclusions Angiogenesis had a high relationship with the K-ras mutations, and may be the early events in the development and pathogenesis of PAC, perhaps play an important role in the progression from CP to PAC.

关 键 词：慢性胰腺炎 胰腺癌 血管生成 诊断 免疫组织化学 RAS基因产物 P21蛋白 

分 类 号：R735.9[医药卫生—肿瘤]