兔晶状体上皮细胞的体外培养  

Cell Culture of Rabbit Lens Epithelium in Vitro

作　　者：黄蓉[1] 袁南荣[2] 管怀进[3] 胡楠[3] 

机构地区：[1]南京医科大学 [2]南京医科大学附属医院眼科 [3]南通医学院附属医院,226001

出　　处：《交通医学》2002年第5期499-500,共2页Medical Journal of Communications

摘　　要：目的 :建立兔晶状体上皮细胞 (RLEC)体外培养的模型。方法 :采用组织块培养法 ,对兔晶状体前囊膜进行培养 ,并利用形态学检查方法鉴定。流式细胞仪检测细胞周期。结果 :组织块接种 72小时后可见细胞生长 ,且保持上皮细胞形态 ,12～ 14天细胞融合。体外传代 5代以后 ,细胞呈纤维化生长。细胞周期分析提示体外培养的RLEC保持正常的增殖能力。结论 :组织块培养法能在体外成功培养兔晶状体上皮细胞 ,可用于白内障术后前囊膜及后囊膜混浊发生机制的研究。Objective:To establish a model of cultured rabbit lens epithelium. Methods:Rabbit lens anterior capsule were cultured in vitro with tissue culturing method. The cultured cells were identified by morphological examination and flow cytometry was used to analyze the cell cycle. Results:The lens epithelial cells moved from tissue after 72 hours and maintained typical epithelial morphology.About 12～14 days late the cell confluenced. After passage 5 cells transformed to fibroblasts.Conclusions:We have successfully cultured a cell line of rabbit lens epithelium and it may be utilized in the study of pathogenesis of after-cataract.

关 键 词：晶状体 上皮细胞 细胞培养 RLEC 组织块培养法 细胞周期 

分 类 号：R－33[医药卫生]