P 53基因抑制人肝癌细胞的生长  

SUPPRESSION OF HUMAN HEPATIC CARCINOMA CELL GROWTH BY WILD—TYPE P53

作　　者：刘超亭 曹宇清[1] 范育虹 李岱宗[1] 顾健人[1] 

机构地区：[1]上海市肿瘤研究所生化和分子生物学室 [2]复旦大学生物系

出　　处：《肿瘤》1991年第5期197-198,共2页Tumor

摘　　要：P53基因定位于人17号染色体短臂,正常的P53基因作为一种细胞增殖的抑制因子在正常的细胞中调节生长和分化,但若基因突变或丢失,就使这种调节作用丧失。目前,巳有大量报道,在人脑、乳房、肺、骨、结肠、直肠的恶性肿瘤里,均发生P53基因的突变和野生型P53等位基因经常伴随着丢失。这些研究提示正常的(野生型的)P53产物可能起着抑制瘤生长的作用,而突变或丢失。Deletions of P53 gene occurred commonly in human bepatic carcinomas,suggestingthat the wild-type gene may act as a suppressor of hepatic carcinoma cell growth. Totest this hypothesis, wild-type human P53 genes were transfected into human hepato-cellular carcinoma cell line PLC/PRF/5 .The transfected cell showed tenfold less colony-forming efficiency than the control group. The state of P53 gene in those colonies thatdid form after wild-type transfection needs further study.

关 键 词：P53基因 肝肿瘤 癌细胞 生长 

分 类 号：R735.702[医药卫生—肿瘤]