甜菜夜蛾核型多角体病毒vp39基因重组真核表达质粒的构建及鉴定  

Construction and identification of the recombinant eukaryotic expression plasmid pGEM-IE1-Se39

作　　者：段媛媛[1] 杨成丽[1] 周建刚[1] 范文韬[1] 彭建新[1] 江月[1] 刘德立[1] 

机构地区：[1]华中师范大学生命科学学院,武汉430079

出　　处：《华中师范大学学报（自然科学版）》2002年第3期345-347,352,共4页Journal of Central China Normal University：Natural Sciences

基　　金：国家自然科学基金资助项目 ( 30 170 0 11);国家教育部高等学校骨干教师资助计划 (GG -180 - 10 5 11- 10 10 ) .

摘　　要：利用DNA重组技术 ,将杆状病毒极早期基因ie1启动子基因克隆至重组质粒pGEM Se39中 ,成功地构建了重组真核表达质粒pGEM IE1 Se39.The baculovirus immediately early gene ie1 promoter was subcloned into recombinant plasmid pGEM-Se39 by using DNA recombinant technique. After transformation, the recombinant plasmid was isolated and digested with different endo-restriction enzymes. The result showed that the recombinant eukaryotic expression plasmid pGEM-IE1-Se39 containing an ie1 promotor was constructed successfully.

关 键 词：甜菜夜蛾 核型多角体病毒 vp39 基因重组 真核表达质粒 iel启动子 核衣壳蛋白基因 基因克隆 pGEM-IE1-Se39 

分 类 号：Q782[生物学—分子生物学]