六个人肾癌细胞系的建立及生物学特性的研究  

Establishment and biological characteristics of six human renal cell carcinoma cell lines

作　　者：万云霞[1] 马建辉[1] 陈毓仙[1] 李艳芬[1] 张学斌[1] 石卫[1] 李秀琴[1] 储大同[1] 

机构地区：[1]中国医学科学院肿瘤医院肿瘤研究所生物治疗协作组,北京100021

出　　处：《中华泌尿外科杂志》2002年第9期521-523,共3页Chinese Journal of Urology

摘　　要：目的　建立人肾癌细胞系 ,研究肾癌细胞表型及其肿瘤相关抗原的表达。　方法　病理证实的 6例肾癌原始新鲜肿瘤组织经体外原代和传代培养至稳定生长 ,检测癌细胞集落形成、染色体及裸鼠体内移植瘤生长 ;免疫荧光染色和流式细胞仪分析细胞系细胞表型 ;RT PCR检测MN/CA9基因表达。　结果　建立的 6个细胞系包括透明细胞型 4个 ,透明 嗜色细胞混合型 1个和乳头状腺癌 1个。 6个细胞系均表现恶性肿瘤细胞的生物学特性。 6个细胞系均高表达HLA ClassⅠ和HER2 /neu分子 ;2个弱表达HLA ClassⅡ ;1个高表达CD86 ;均不表达CD80。 3个细胞系表达MN/CA9基因。　结论　新建立的肾癌细胞系可作为研究人肾癌细胞的生物学特性、肿瘤相关抗原。Objective To establish human renal cell carcinoma (RCC) cell lines,and to investigate the cell phenotypes and expression of tumor associated antigens. Methods Fresh tumor tissues from pathologically proven human RCCs were primary cultured and passed generation to generation until a stably grow in vitro.The cell cloning form,chromosome and graft into nude mice in vivo were examined for cell lines.Immunofluorescent stain and flow cytometric analysis were carried out for cell's phenotype,RT PCR examination for MN/CA9 gene expression. Results Six human RCC cell lines have been established,including four of the cell lines derived from clear cells,one from mixed clear granular cells and one from papillary cells.All the cell lines showed the characteristics of malignant cells.All the lines highly expressed HLA ClassⅠ and HER2/neu.Three lines weakly expressed HLA ClassⅡ and one cell line highly expressed CD86 but all the lines did not express CD80.RT PCR showed that three cell lines have the expression of MN/CA9 gene. Conclusions These newly established RCC cell lines would provide a useful in vitro model for studies related to biological characteristics,tumor associated antigen,immunogenity and immunotherapy of human RCC.

关 键 词：肾癌细胞系 细胞表型 肿瘤相关抗原 免疫荧光染色 流式细胞仪 生物学特性 

分 类 号：R737.11[医药卫生—肿瘤]