去蛋氨酸肠内营养联合阿霉素对大鼠Walker-256癌肉瘤同步化疗的研究  

Synergistic Anticancer Effect of Adriamycin and Methionine-depleted Enteral Nutrition on Walker-256Carcinosarcoma in Wistar Rats

作　　者：贺银成[1] 黎文[2] 郑科炎[1] 刘权焰[1] 曹军[1] 

机构地区：[1]武汉大学中南医院普外科 [2]<临床外科杂志>编辑部

出　　处：《中国临床营养杂志》2002年第3期169-171,共3页Chinese Journal of Clinical Nutrition

摘　　要：目的探讨蛋氨酸缺乏对阿霉素抗肿瘤效应的协同作用。方法SD大鼠空肠造瘘,皮下接种Walker-256癌肉瘤,分A组(平衡氨基酸+生理盐水组)、B组(平衡氨基酸+阿霉素组)、C组(去蛋氨酸+阿霉素组)3组,测定各组治疗后肿瘤细胞周期、细胞核DNA含量、荷瘤鼠生存时间等。结果C组G0/G1细胞百分数犤(68.5±5.3)%犦高于A、B组犤(50.6±6.0)%及(51.9±5.6)%犦,S期和G2M期百分数及增殖指数明显降低;B、C组抑瘤率分别为28.8%及42.4%(P<0.05)。C组荷瘤大鼠生存时间明显延长。结论蛋氨酸缺乏可使肿瘤细胞阻滞于晚S/G2期,增强阿霉素的抗肿瘤效应。Objective To study synergistic anticancer effect of adriamycin(ADM)and methionine-free enteral nutrition on Walker-256carcinosarcoma in Wistar rats.Methods The SD rats were each given a catheteriza tion of jejunostomy and an inoculation of Walker-256carcinosarcoma cells subcutaneously.Thirty-six rats were randomized into3groups:group A (Met + +NS),B (Met + +ADM)and C(Met - +ADM).The tumor size,tumor inhibitory rate,cell cycle,DNA contents and the life span were investigated.Results The rate of G 0 /G 1 in group C(68.5±5.3)% was higher than those in group A and B (50.6±6.0)%,(51.9±5.6)(P<0.05).The rates of S/G 2 M and proliferation index(PI)in group C were lower than those in group A and B.The inhibitory rates of tumor in group B and C were28.8%and42.4%respectively(P<0.05).The life span in group C was significantly prolonged than those in group A and B.Conclusions The methionine starvation,may arrest tumor cells in the late S/G 2 phase of cell cycle,and the antitumor effect of adriamycin may be enhanced.

关 键 词：Walker-256癌肉瘤 蛋氨酸 阿霉素 肿瘤 肠内营养 大鼠 药物疗法 治疗 

分 类 号：R730.53[医药卫生—肿瘤]