早老龄小鼠脑缺血再灌后能量代谢障碍及聪圣胶囊对其影响  

Disorder of Energy Metabolism Caused by Cerebral Ischemia and Reperfusion in Presenile Mice: the Effects of Congsheng Capsules

作　　者：赵玲[1] 徐秋萍[1] 李林[2] 

机构地区：[1]北京中医药大学,北京100029 [2]首都医科大学宣武医院药理研究室北京脑老化重点实验室,北京100053

出　　处：《北京中医药大学学报》2002年第5期14-16,共3页Journal of Beijing University of Traditional Chinese Medicine

基　　金："九五"国家科技攻关课题 (No .96 90 6 0 9 0 3)

摘　　要：采用早老龄小鼠双侧颈总动脉反复缺血再灌合并尾部放血的方法造成脑缺血模型 ,用高效液相层析法 (HPLC)测定脑匀浆中三种腺苷酸含量计算出脑能量负荷。分别观察聪圣胶囊对脑组织能量负荷、乳酸 (LA)、乳酸脱氢酶 (LDH)的影响。结果 :聪圣胶囊 3、9g kg可明显改善能量负荷 ;1、3、9g kg可明显减轻模型小鼠乳酸酸中毒程度 ,表现为LA含量减少 ,LDH活力提高。结论 :聪圣胶囊可通过改善能量代谢 ,减轻脑乳酸酸中毒程度 。The cerebral ischemia model was established by repeated ischemia and reperfusion of common carotid arteries and tail bloodletting in the presenile mice. The contents of 3 kinds of AMP in the mouse brain homogenate were determined by HPLC and the brain energy charge was calculated. The effects of Congsheng Capsules (CC) on the brain energy charge and the levels of LA and LDH were observed. The results showed that doses of 3 g /kg and 9 g/kg of CC could markedly improve the brain energy charge, and doses of 1 g/kg, 3 g/kg and 9 g/kg of CC could remarkably decrease the level of LA and increase the activity of LDH in the model mice.

关 键 词：小鼠 脑缺血 聪圣胶囊 能量代谢 乳酸 

分 类 号：R285.5[医药卫生—中药学] R277.733.1[医药卫生—中医学]