鼠脑局部缺血再灌注的形态学与细胞凋亡的研究  

A study of morphological change of ischemia reperfusion and cell apoptosis in rat brain

作　　者：谭军[1] 杨廷桐[2] 范琦芳[1] 

机构地区：[1]新乡医学院三附院神经内科,河南新乡453003 [2]新乡医学院病理教研室

出　　处：《新乡医学院学报》2002年第4期260-262,共3页Journal of Xinxiang Medical University

摘　　要：目的　探讨大脑局部缺血再灌注的形态学变化与脑细胞凋亡的关系。方法　建立鼠大脑缺血再灌注的模型 ,采用常规染色及TTC、Thionine染色和凋亡检测。结果　假手术组大脑无病理形态改变 ,阻塞组和复通组在术后 48h病理形态相似 ,8～ 15d后复通组比阻塞组有明显的病灶缩小、修复等现象 (P <0 .0 1) ,复通组交界区凋亡细胞数量明显减少。结论　脑缺血后的即早灌注有利于病情晚期的恢复 。Objective To study the relationship between morphological changes of ischemia reperfusion and cell apoptosis in rat brain and to prove theoretic basis for treating cerebral ischemia. Methods A model with ischemia-reperfusion was established in rat brain by HE,TTC,Thionine and TUNEL dyeing.Results There was no morphology change and clinical meaning in sham operated group. Similar pathological change in permanent occluded and reperfussed group was found 48h after operation.Pathological repair was more remarkable in the reperfused than the occluded group 8 days after operation.The number of apoptosis cell was decreased on boundary in reperfused .Conclusion Reperfusion after ischemia is advantageous to the recovery of the cerebral ischemia in the later stage,but at the same time it may lead to slight damage of nervous cells.

关 键 词：脑局部缺血 缺血再灌注 细胞凋亡 形态学 大鼠 

分 类 号：R743.31[医药卫生—神经病学与精神病学]