大鼠肝内移植肿瘤与肥大细胞关系的形态学研究  

Relationship between morphology of mast cells and tumor transplanted in rat liver

作　　者：阮幼冰[1] 官阳[1] 张志勇[1] 

机构地区：[1]华中科技大学同济医学院病理学系,武汉430030

出　　处：《临床与实验病理学杂志》2002年第4期393-396,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家自然科学基金资助项目(No39970336)

摘　　要：目的 :研究大鼠肝内移植肿瘤与其周边肥大细胞的关系。方法 :建立 4 0只雄性Wistar大鼠肝内移植肿瘤模型 ,运用HE染色 ,肥大细胞 (mastcell,MC)Alcianblue特殊染色 ,苦味酸 -天狼猩红染色和电镜等技术 ,观察移植肿瘤肝组织的形态学改变 ,肿瘤周边浸润MC的数量以及MC与肿瘤组织的关系 ,8只正常大鼠为对照组。结果 :肿瘤组织周边部分肝细胞形态学异常 ;肝组织内胶原纤维增生 ,主要为Ⅰ型和Ⅲ型胶原 ,并包绕肿瘤组织 ,肿瘤周边MC浸润 ,并沿胶原纤维分布 ,不同大鼠其肿瘤周边MC数量不一 ,有的差异明显。超微结构观察显示MC通过多个接触点与肿瘤细胞紧密相连 ,并释放胞质颗粒内容物 ,导致肿瘤细胞崩解。结论 :MC与肿瘤组织有着非常密切的关系 ,MC可能通过多种途径直接和间接地发挥抗肿瘤作用目的 :研究大鼠肝内移植肿瘤与其周边肥大细胞的关系。方法 :建立 4 0只雄性Wistar大鼠肝内移植肿瘤模型 ,运用HE染色 ,肥大细胞 (mastcell,MC)Alcianblue特殊染色 ,苦味酸 -天狼猩红染色和电镜等技术 ,观察移植肿瘤肝组织的形态学改变 ,肿瘤周边浸润MC的数量以及MC与肿瘤组织的关系 ,8只正常大鼠为对照组。结果 :肿瘤组织周边部分肝细胞形态学异常 ;肝组织内胶原纤维增生 ,主要为Ⅰ型和Ⅲ型胶原 ,并包绕肿瘤组织 ,肿瘤?Purpose To study the relationship between morphology of mast cells (MCs) and tumor transplanted in rat liver. Methods Forty male Wistar rats were used in the tumor models, and 8 rats as normal control. The authors investigated the morphological change of liver tissue, the number of MCs around the tumor and relationship between MCs and tumor by using H E, MC Alcian blue, picric acid and sivius red stains and electronic microscopy. Results Hepatocytes around the tumor had abnormally morphological changes. There were a number of collagenous deposition around the tumor, mainly type Ⅰ and Ⅲ collagens. MCs infiltrated around the tumors and distributed along collagenous fibrils. There were a difference in MCs number around the tumor in different rats, and sometimes the difference were very significant. Ultrastructural observation showed that MCs contacted closely with the tumor cells. The content inside the granules were discharged and spread along the intercellular space between the tumor cells. And tumor cells were killed. Conclusions There exists a very close relationship between tumor tissue and MCs. MCs may inhibit tumor cells by several direct and indirect pathways.

关 键 词：形态学研究 肝肿瘤 肥大细胞 胶原纤维 大鼠 

分 类 号：R735.7[医药卫生—肿瘤]