栉孔扇贝16S rRNA基因片段序列的多态性研究  

SEQUENCE POLYMORPHISM OF MITOCHONDRIAL 16S rRNA GENE FRAGMENT IN SCALLOP CHLAMYS FARRERI

作　　者：刘亚军[1] 喻子牛 姜艳艳[1] 张留所 孔晓瑜[1] 宋林生[1] 

机构地区：[1]青岛海洋大学教育部海水养殖重点实验室,青岛266003 [2]中国科学院海洋研究所,青岛266071

出　　处：《海洋与湖沼》2002年第5期477-483,共7页Oceanologia Et Limnologia Sinica

基　　金：国家重点基础研究发展规划项目 (973 )资助G1 9990 1 2 0 0 8号;国家自然科学基金资助项目 3 960 0 1 1 3号

摘　　要：采用PCR技术扩增了栉孔扇贝 (Chlamysfarreri)线粒体DNA的 1 6SrRNA基因片段 ,PCR产物经T载体连接之后进行了克隆、测序 ,得到 634bp的核苷酸序列 ;分析了该片段的大连、烟台、青岛、朝鲜 4个自然群体 31个个体的核苷酸序列多态性 ,共检测到 2 6个多态性核苷酸位点、1 8种单倍型。结果表明 ,4个群体中以朝鲜群体遗传多样性最高 ,其次为烟台、青岛群体 ,大连群体最低 ;DNA sequencing is very useful for the genetic diversity analysis at molecular level. Mitochondrial 16S rRNA gene fragment of Scallop Chlamys farreri was amplified via polymerase chain reaction (PCR), the PCR products were ligated into T vector, cloned and sequenced for 31 individuals, 3 populations of China (8 for Dalian and Qingdao each, and 7 for Yantai) and one population of Republic of Korea. 634 base pair nucleotide sequences were examined and analyzed for genetic polymorphism. Resultant sequences data analysis showed that all 31 sequences were grouped into 18 haplotypes and there were 26 variable nucleotide positions in this gene fragment, which included transitions and transvertions, and no insertion/deletion were observed. The genetic diversity indexes including S (polymorphic sites), P (nucleotide diversity) and K (average number of nucleotide difference) were calculated and the results indicated that Korea population showed more variation than others and more differences to others, followed by Yantai and Qigndao population, and Dalian population had the least sequence variation. Parameters of genetic variation among the four populations suggested that no significant genetic difference was observed. Our data provided baseline information at DNA level on the genetic variation in wild Scallop populations and may be useful for future management and utilization of the resource.

关 键 词：栉孔扇贝 线粒体DNA 16SRRNA基因 DNA序列测定 PCR技术 克隆 核苷酸序列 多态性 

分 类 号：Q959.21[生物学—动物学] S968.313[农业科学—水产养殖]