血管形成抑制剂TNP-470对人卵巢癌裸鼠皮下移植瘤生长的影响  

Experimental therapy with angiogenesis inhibitor TNP-470 on human ovarian cancer transplanted subcutaniously in nude mice

作　　者：孔海宏[1] 金志军[2] 姚明[3] 

机构地区：[1]解放军第四一一医院妇产科,上海200081 [2]第二军医大学长征医院妇产科 [3]上海市肿瘤研究所

出　　处：《中华妇产科杂志》2002年第9期556-558,共3页Chinese Journal of Obstetrics and Gynecology

摘　　要：目的　观察血管形成抑制剂TNP 470 (简称TNP)对人卵巢癌裸鼠皮下移植瘤生长的影响。方法　对 3 0只裸鼠建立人卵巢癌皮下移植瘤模型 ,共分为 5组 :对照组 (皮内及腹腔注射无菌生理盐水 )、溶剂组 (皮内注射 1%乙醇及 5 %阿拉伯糖混合液 )、TNP组 (皮内注射TNP)、环磷酰胺 (CTX)组 (腹腔注射CTX)、TNP +CTX组 (皮内注射TNP ,同时腹腔注射CTX)。观察各组裸鼠皮下移植瘤生长情况、裸鼠体重的变化 ,计算抑瘤率 ,并进行病理学检查。结果　溶剂组抑瘤率为 7 9% ,对照组为0 % ,两组比较 ,差异无显著性 (P >0 0 5 ) ;TNP组抑瘤率为 2 6 1% ,CTX组为 3 3 9% ,两组比较 ,差异无显著性 (P >0 0 5 ) ;TNP +CTX组抑瘤率高达 70 5 % ,有极明显的抑瘤效应 ,与对照组比较 ,差异有极显著性 (P <0 0 0 5 )。TNP +CTX组移植瘤镜下可见肿瘤细胞核浆比例明显小于对照组 ,核异形性明显少于对照组。结论　TNP对卵巢癌有一定的治疗作用 ;TNP与化学药物联合应用 。Objective To study the effect of an angiogenesis inhibitor TNP-470 (TNP )used alone and in combination with cytoxan(CTX) in the treatment of human ovarian cancer transplanted s.c. in nude mice. Methods Human ovarian cancer transplanted s.c. in nude mice model was established, then divided into 5 groups: control group, vehicle group, TNP group,CTX group and TNP+CTX group, different treatments were served from day 8 after transplantation and all mice were sacrificed after 28 days. The weights of the mice and the volumes of the tumors were measured respectively during the therapy time. Moreover, microscopy was done after H&E staining. Results The growth inhibiting rates in the TNP and CTX group were 26.1% and 33.9% respectively; After combined, the rate was increased to 70.5%. There were no obvious decrease in the weight of all treated mice. Conclusions Treatment with TNP is an potentially useful method of antitumor therapy in ovarian cancer, although the inhibition effects were not obvious in small doses. Moreover,TNP could enhance the effectiveness of antitumor drug.

关 键 词：卵巢癌 血管生成抑制剂 倍半萜类 肿瘤移植 

分 类 号：R737.31[医药卫生—肿瘤]