As_2O_3逆转K_(562/ADM)细胞多药耐药的研究  

Reversal Effect of As_2O_3 on Multidrug Resistance K_(562/ADM) Cell Line

作　　者：王淑婷[1] 李清[1] 刘超[1] 赵谨瑶[2] 于丽敏[2] 卢步峰[2] 杨佩满[2] 

机构地区：[1]大连大学医学院,辽宁大连116622 [2]大连医科大学省细胞分子生物学重点实验室,辽宁大连116027

出　　处：《解剖科学进展》2002年第3期237-239,共3页Progress of Anatomical Sciences

摘　　要：目的　阐述As2 O3 (三氧化二砷 )治疗人红白血病机制。方法　采用人红白血病多药耐药株K562 / ADM作为实验模型 ,研究As2 O3 对该细胞MDR逆转的可能性。结果　①K562 / ADM 细胞对ADM具有明显的抗性 ,与K562 细胞相比 ,抗性倍数约为 4 0倍 ,而两者对As2 O3 的IC50 接近 ,无显著差异 ;②低毒剂量的As2 O3 (1 0 μmol/L)与ADM(4 0 μg/ml)联合运用 ,可升高细胞内ADM的浓度 ,降低细胞对ADM的IC50 ,使该细胞对ADM的抗性有数倍逆转。结论　As2 O3 是一种有效的MDR逆转剂。Objective To illustrate mechanisms of arsenic trioxide (As 2O 3) in the treatment of K 562 / ADM cells. Methods We study the reversal effect of As 2O 3 on K 562 / ADM MDR cells in experiment model of rat. Results ① The resistance of K 562 / ADM cell line to ADM was 40 times than that of K 562 cell line, but the IC 50 value of both cell lines to As 2O 3 was similar (P>0.05); ② low cytotoxic dose (2.0 μmol/L) of As 2O 3 in combination with ADM (4.0 μg/ml) increased the intracellular ADM concentration (P<0.05) and declined IC 50 value of K 562 / ADM cell line to ADM by 2.5 times than the control. Conclusion As 2O 3 might be an effective anti tumor drug as well as an effective drug for reversal of MDR.

关 键 词：三氧化二砷 多药耐药性 人红白血病细胞 抗瘤作用 P-糖蛋白 荧光分光光度法 

分 类 号：R733.73[医药卫生—肿瘤] R965[医药卫生—临床医学]