阿尔茨海默病海马CA1区p75^(NTR)和磷酸化tau蛋白神经元的定量观察  

Quantitative analysis of p75 ^( NTR) and hyperphosphorylated tau containing neurons in the hippocampal CA1 subfield of Alzheimer disease

作　　者：胡祥友[1] 许浩[1] 周江宁[1] 

机构地区：[1]中国科学技术大学生命科学学院神经生物系,合肥230026

出　　处：《中华神经科杂志》2002年第4期198-200,共3页Chinese Journal of Neurology

基　　金：国家自然科学基金资助项目 ( 30 0 70 82 6 )

摘　　要：目的　观察阿尔茨海默病 (AD)海马CA1区p75 NTR的表达与磷酸化tau蛋白的关系。方法　利用组织化学和免疫细胞化学方法分析由荷兰脑库提供的 10例女性AD患者及年龄、性别等与之匹配的 10例健康意外死亡对照者的脑组织标本海马CA1区神经元总数、p75 NTR神经元数目的差异及AD患者p75 NTR和Alz 5 0共存。结果　AD患者海马CA1区神经元总数明显低于对照组 ,但p75 NTR神经元占总神经元的百分比明显高于对照组 (P值分别为 0 .0 0 0 2、0 .0 0 1) ;AD海马CA1区Alz 5 0阳性神经元 (个 /mm2 )为 87.5± 2 9.2 ,p75 NTR和Alz 5 0双标神经元为 76 .4± 2 6 .6 ,后者占前者的百分比为86 6 %± 5 .0 % ,二者在CA1区的分布呈正相关 (r =0 .79、P =0 .0 0 6 )。结论　AD患者海马CA1区p75 NTR主要产生死亡信号 ,参与AD神经病理的形成。Objective To investigate p75 NTR expressing neurons and hyperphosphorylated tau protein containing neurons in the hippocampal CA1 subfield of Alzheimer's disease. Methods Samples of hippocampus of 10 female AD patients and 10 non-demented female controls matched with age and postmortem delay were obtained from the Netherlands Brain Bank. The main body of hippocampus was dissected, dehydrated and embedded in paraffin. Serial 6-μm coronal sections were cut, and 3 successive sections were selected. The first section was stained with 0.5% thionin, the second was processed for p75 NTR immunocytochemistry and the third was processed for p75 NTR double-labeling immunocytochemistry with Alz-50. For quantitative analysis of the total number of neurons, p75 NTR expressing neurons and neurons colocalizing p75 NTR and Alz-50 in the CA1 subfields, a MetaMorph image acquisition and processing software (Universal Imaging Corp, USA) was used.Results The total number of neurons, p75 NTR immunoreactive neurons and ratio of the latter to the former in 1 mm2 of the CA1 subfield of AD patients were 293.2±37.0, 116.0±20.4 and 39.7%±5.3%, respectively, of controls were 473.6±63.1, 136.7±24.4 and 28.9%±3.7%, respectively. There was significant decrease in the total number of neurons of the AD patients in comparison with controls (P=0.000 2). However, the ratio of p75 NTR neurons to total number of neurons was significant increase in AD patients compared with controls (P=0.001). The number of Alz-50 neurons, Alz-50 and p75 NTR double-labeling neurons and ratio of the latter to the former in 1 mm2 of the CA1 subfield of AD patients were 87.5±29.2, 76.4±26.6 and 86.6%±5.0%, respectively. Furthermore, the number of p75 NTR containing neurons was significantly correlated to the number of Alz-50 expressing neurons (r=0.79, P=0.006). Conclusion The p75 NTR may interact with hyperphosphorylated tau protein and involve in the pathogenesis of Alzheimer′s disease.

关 键 词：阿尔茨海默病 海马CA1区 P75^NTR 磷酸化TAU蛋白 神经元 免疫组织化学 

分 类 号：R749.1[医药卫生—神经病学与精神病学]