S100 A2基因cDNA克隆及其在肝癌细胞中的表达  

Cloning and Expression S100A2 Gene cDNA in Hepatocellular Carcinoma Cells

作　　者：王秀清[1] 陈亦欣[1] 徐敏[1] 白桦[1] 郑瑾[1] 陈伟[1] 刘红建[2] 

机构地区：[1]暨南大学医学院第二附属医院,深圳518020 [2]吉林大学再生医学科学研究所,长春130021

出　　处：《现代临床医学生物工程学杂志》2002年第4期244-245,共2页Journal of Modern Clinical Medical Bioengineering

基　　金：深圳市科技局资助项目 (编号 :2 0 0 0 0 4 0 58)

摘　　要：目的　克隆S10 0A2cDNA、构建S10 0A2重组表达载体并在肝癌细胞QGY770 1中进行表达 .方法　应用RT-PCR和DNA重组技术构建绿色荧光蛋白 (EGFP) -S10 0A2融合蛋白表达载体 ,经脂质体导入QGY770 1细胞中进行表达 .应用荧光显微镜直接观察EGFP -S10 0A2融合蛋白的表达情况 ,G4 18筛选阳性细胞克隆 .结果　构建了带有EGFP的S10 0A2重组表达载体命名为EGFP -C2 -A2 ,并在肝癌细胞QGY770 1中获得了表达 .荧光显微镜下可见EGFP-S10 0A2融合蛋白定位于胞浆及胞核 ,而pEGFP载体对照之EGFP只定位在胞浆中 .结论　EGFP -S10 0A2融合蛋白能够在肝癌细胞QGY770 1中获得表达 ,各自在细胞中的定位互不受影响 ,推断此融合蛋白具有各自的生物学活性 .Objective To express the S100A2 protein in hepatocellular carcinoma cells. Methods We constructed the recombinant plasmid of enhanced green fluorescent protein (EGFP) and S100A2 by RT-PCR and DNA recombinant technique, and introduced into the cells with lipofectin TM . The expression and location of the products were observed by fluorescent microscopy. The positive cell clones were selected by adding G418 reagent. Results The recombinant plasmid of EGFP and S100A2 was constructed and named as pEGFP-C2-A2. The products of pEGFP-C2-A2 were detected and appeared to be localized in the cytoplasm and nucleus. While the pEGFP control products were found to be localized only in the cytoplasm. Conclusions the chimera protein of EFGP-S100A2 can be expressed in hepatocellular carcinoma cells. It is suggested that the chimera protein may possess the functions of EGFP and S100A2 respectively.

关 键 词：S100A2基因 CDNA克隆 肝癌细胞 基因表达 肿瘤生物学 

分 类 号：R735.7[医药卫生—肿瘤]