测定卵巢恶性肿瘤患者血清DJ-1蛋白的临床价值  

Elevated serum level of DJ-1 in malignant ovarian tumor and its clinical significance

作　　者：曾志英[1] 周杰[2] 张群锋 刘珏 黄余良 

机构地区：[1]南华大学附属第二医院麻醉科,衡阳421001 [2]南华大学附属第二医院妇产科,衡阳421001

出　　处：《现代妇产科进展》2016年第6期429-432,436,共5页Progress in Obstetrics and Gynecology

基　　金：湖南省教育厅基金项目(No:15C1211)

摘　　要：目的:探讨血清中DJ-1蛋白浓度与卵巢恶性肿瘤的关系,以及检测DJ-1蛋白在卵巢肿瘤诊断中的价值。方法:ELISA法测定62例健康妇女(对照组)、60例卵巢良性肿瘤患者(良性组)及138例卵巢恶性肿瘤患者(恶性组)血清中DJ-1蛋白含量。结果:恶性组治疗前平均血清DJ-1蛋白水平[(9.64±4.06)ng/ml]显著高于良性组[(4.87±1.86)ng/ml]和对照组[(4.35±1.44)ng/ml](P=0.000)。恶性组术后和复发组的平均血清DJ-1蛋白水平分别为[(8.62±3.60)ng/ml]和[(9.46±2.98)ng/ml],较对照组及良性组有明显升高,差异有统计学意义(P=0.000)。恶性治疗前及术后配对组中,患者治疗前的平均血清DJ-1蛋白水平[(9.12±3.92)ng/ml]高于术后组[(8.46±3.75)ng/ml](P=0.022)。血清DJ-1蛋白阳性患者的累积生存率与血清DJ-1蛋白阴性者比较,差异有统计学意义(P=0.028)。COX模型结果显示,DJ-1蛋白水平、临床分期及残余灶大小是独立的预后因素(P<0.05)。结论:卵巢恶性肿瘤患者中血清DJ-1蛋白含量异常升高,DJ-1蛋白可协助卵巢恶性肿瘤的诊断,同时对患者手术效果的评价、判断预后及复发也提供了相应的参考价值。Objective: To discuss the relationship between the serum Level of DJ-1 in patients with malignant ovarian tumors,and to explore the clinical value of detection in diagnosis of malignant ovarian tumors. Methods: The concentration of serum DJ-1 in 62 healthy controls,138 patients with malignant ovarian tumors and 60 patients with benign ovarian tumors were measured by ELISA. Results: The concentration of serum DJ-1 in patients with malignant ovarian tumors before treatment [( 9. 64 ± 4. 06) ng / ml]were obviously higher than those in healthy group[( 4. 35 ±1. 44) ng / ml]and patients with benign ovarian tumor[( 4. 87 ±1. 86) ng /ml]( P = 0. 000). The concentration of serum DJ-1 in postoperative patients[( 8. 62 ±3. 60) ng /ml]and patients with recurrence[( 9. 46 ±2. 98) ng / ml]were also obviously higher than those in healthy group and patients with benign ovarian tumor( P = 0. 000). In the matched-pairs group,the concentration of serum DJ-1 in patients before treatment [( 9. 12 ± 3. 92) ng / ml]were also significantly higher than those in patients[( 8. 46 ±3. 75) ng / ml]( P = 0. 022). Comparison of serum DJ-1 positive patients the cumulative survival rate and serum DJ-1 negative patients,the difference was significant( P = 0. 028),the COX model results show that the DJ-1 level,clinical stage and residual lesion size were independent prognostic factors( P<0. 05). Conclusions: Patients with malignant ovarian tumor appear serum DJ-1 level increased and the DJ-1 might be used to assist the diagnosis of ovarian malignant tumors. At the same time,the DJ-1 might be used to evaluate the response to operation,judge prognosis and recurrence in patients of the malignant ovarian tumor.

关 键 词：DJ-1蛋白 卵巢恶性肿瘤 血清 酶联免疫吸附法 

分 类 号：R737.31[医药卫生—肿瘤]