miR-17～92基因簇过表达调控PTEN、BIM蛋白对卵巢癌SKOV3细胞紫杉醇耐药的影响  

Overexpression of miR-17 ~ 92 cluster effect the paclitaxel chemoresistance of ovarian cancer SKOV3 cells through PTEN、BIM protein

作　　者：接智慧[1,2] 吴建磊[1,2] 王敏[2] 

机构地区：[1]赤峰学院附属医院妇产科,赤峰024005 [2]中国医科大学附属盛京医院妇产科,沈阳110004

出　　处：《现代妇产科进展》2016年第7期507-510,共4页Progress in Obstetrics and Gynecology

摘　　要：目的:分析miRNA-17~92基因簇过表达与卵巢癌SKOV3细胞紫杉醇耐药的关系,为卵巢癌化疗耐药的研究提供理论依据。方法:脂质体法将构建的p EGFP-N1-miR-17~92质粒转染入卵巢癌SKOV3细胞。Real time-PCR法及Western blot法分别检测转染前后miR-17~92及BIM、PTEN表达。结果:与SKOV3细胞相比,空质粒p EGFPN1转染的SKOV3-p EGFP-N1细胞miR-17~92含量0.970,与对照组比较差异无统计学意义(t=0.581,P=0.620);过表达质粒p EGFP-N1-miR-17~92转染的SKOV3-p EGFP-N1-miR-17~92细胞miR-17~92含量2.082,与对照组比较差异有统计学意义(t=-5.602,P=0.030);SKOV3-p EGFP-N1-miR-17~92细胞组的miR-17~92 mRNA相对表达量与SKOV3-p EGFP-N1细胞组比较,差异有统计学意义(t=-8.473,P=0.014)。不同浓度紫杉醇作用于转染p EGFP-N1-miR-17~92质粒后细胞,miR-17~92过表达使BIM蛋白表达下降,对PTEN蛋白表达的影响不明显。结论:miR-17~92基因簇与卵巢癌SKOV3细胞紫杉醇耐药有关。miR-~92基因簇过表达影响卵巢癌耐药的机制是通过下调BIM蛋白而非PTEN蛋白参与。Objective: To investigate the relationship between miRNA-17 ~ 92 cluster and the paclitaxel chemoresistance of ovarian cancer,provided a theoretical basis for the chemoresistance of ovarian cancer. Methods: Using liposome method transfected p EGFP-N1-miRNA-17 ~ 92 plasmid into ovarian cancer SKOV3 cells. The expressions of miR-17 ~ 92,BIM,PTEN were detected using Real time-polymerase chain reaction( Real time-PCR) and Western blot. Results: Compared with the SKOV3 cells,empty plasmid p EGFP-SKOV3-p EGFP-N1 transfection cell miR-17 ~ 92 content was 0. 970,there was no statistically significant difference compared with control group( P>0. 05). The miR-17 ~ 92 levels of SKOV3-p EGFP-N1-miR-17~ 92 cells was 2. 082,and compared with the control group difference was statistically significant( P<0. 05). In SKOV3-p EGFP-N1-miRNA-17 ~ 92 cells and SKOV3-p EGFP-N1 cells,the differences of miR-17 ~ 92 mRNA levels were statistically significant( P<0. 05). Overexpression miRNA- 17 ~ 92 decreased BIM protein expression,the expression of PTEN protein of the effect was not obvious. Conclusions: miRNA-17 ~ 92 gene clusters involved in paclitaxel resistance of ovarian cancer SKOV3 cells. The mechanism of miRNA-17 ~ 92 gene cluster affecting ovarian cancer drug resistance is through cuting BIM protein instead of PTEN protein.

关 键 词：miR-17~92基因簇 卵巢癌 耐药 PTEN BIM 

分 类 号：R737.31[医药卫生—肿瘤]