白血病相关基因MLAA-34在HeLa细胞中的抗凋亡作用  

Antiapoptotic Effect of the Leukemia Associated Gene MLAA-34 in HeLa Cells

作　　者：张鹏宇[1] 赵璇[2] 张文娟[3] 张王刚[1] 陈银霞[1] 

机构地区：[1]西安交通大学第二附属医院血液科,陕西西安710004 [2]西安医学院基础部组胚教研室,陕西西安710021 [3]陕西省血液中心,陕西西安710061

出　　处：《中国实验血液学杂志》2016年第2期363-368,共6页Journal of Experimental Hematology

基　　金：国家自然科学基金项目(81000219);西安交通大学第二附属医院人才培养专项科研基金资助

摘　　要：目的:研究白血病相关基因MLAA-34在He La细胞中的抗凋亡作用。方法:以HeLa细胞株为研究对象,构建MLAA-34重组慢病毒表达载体,建立了高表达MLAA-34的稳定转染Hela细胞株;应用As_2O_3诱导其凋亡,采用MTT法、平板克隆形成实验、流式细胞术分别检测细胞增殖能力、集落形成能力、凋亡及细胞周期分布的变化。结果:应用As_2O_3处理后,稳定转染MLAA-34的Hela细胞株的存活率明显高于对照组,其集落形成能力显著增加;MLAA-34基因高表达显著降低了HeLa细胞的凋亡率,同时相应的降低了G_2/M期细胞的比例。结论:白血病相关基因MLAA-34在HeLa细胞中具有抑制As_2O_3诱导的凋亡作用。Objective:To study the antiapoptotic effect of leukemia- associated gene MLAA-34 in HeLa cells.Methods:The MLAA-34 recombinant lentiviral expression vector was constructed,and the stably transfected HeLa cell line with high expression of MLAA-34 was set up;As_2O_3 was used to induce apoptosis;the MTT assay,colony formation test and flow cytometry were used to detect the ability of cell proliferation,colong formation,apoptosis and cell cycle changes respectively.Results:After treatment with As_2O_3,the survival rate of HeLa cells with MLAA-34 overexpression was significantly higher than that of the control cells,and the colony formation ability of MLAA-34 significantly increased,and the high expression of MLAA-34 gene significantly decreased the apoptosis rate of HeLa cells,and decreased the proportion of G_2/M phase cells.Conclusion:The leukemia- associated gene MLAA-34 has been comfirmed to show antiapoptotic effect in HeLa cells which are induced by As_2O_3.

关 键 词：MLAA-34基因 CAB39L基因 HELA细胞 抗凋亡作用 

分 类 号：R733.7[医药卫生—肿瘤]