血管内皮生长因子抗体对卵巢癌细胞株SKOV3体外生长的影响  

The Effect of Antibody of Vascular Endotheial Growth Factor on Ovarian Cancer Line SKOV3 in vitro

作　　者：李力[1] 王丽梅[1] 张玮[1] 

机构地区：[1]广西医科大学附属肿瘤医院妇瘤科,南宁530021

出　　处：《中国肿瘤生物治疗杂志》2002年第3期190-193,共4页Chinese Journal of Cancer Biotherapy

基　　金：广西壮族自治区科技厅自然科学基金(桂科回0 0 90 0 9)资助

摘　　要：目的 :探讨血管内皮生长因子抗体对卵巢癌细胞株SKOV3体外生长的影响。方法 :采用体外实验的方法将不同浓度的血管内皮生长因子抗体作用卵巢癌细胞系SKOV3。用RT PCR和免疫组化法测定处理前后卵巢癌细胞系SKOV3中血管内皮生长因子mRNA和蛋白的表达。采用ELISA法测定SKOV3分泌血管内皮生长因子的含量 ,采用MTT和细胞直接计数方法测定处理前后卵巢癌细胞系SKOV3的抑制率和增殖速度。结果 :不同浓度的血管内皮生长因子抗体作用于卵巢癌细胞株SKOV3后 ,其生长抑制率和细胞增殖速度与对照相比较 ,无显著性差异 (P >0 .0 5 ) ;不同浓度的血管内皮生长因子抗体作用于卵巢癌细胞株SKOV3后 ,其血管内皮生长因子mRNA、蛋白及其受体表达水平呈下调趋势 ;不同浓度的血管内皮生长因子抗体作用于卵巢癌细胞株SKOV3后 ,其血管内皮生长因子分泌明显下降 (P <0 .0 5 )。结论 :血管内皮生长因子抗体对卵巢癌细胞株SKOV3体外生长无直接影响 ,但血管内皮生长因子抗体可下调血管内皮生长因子mRNA、蛋白及其受体表达水平。Objective: To explore the effect of antibody of vascular endothelial growth factor (VEGF)on ovarian cancer line SKOV3. Methods: The different concentrations of anti VEGF antibody was used to act on SKOV3 in vitro . The RT PCR and immunohistochemically methods were used to determine mRNA and protein expression of VEGF in SKOV3. The VEGF content of SKOV3 in culture medium was test by ELISA. The MTT and cell counting were also used to determine the growth curve and inhibited rate of SKOV3 under different concentrations of anti VEGF antibody. Results: (1) After the different concentrate of anti VEGF antibody was used to act on SKOV3 in vitro , there is not significant difference in inhibition rate and proliferation rate compared with the control ( P >0.05). (2) VEGF mRNA, protein and VEGF receptor expression of SKOV3 treated with anti VEGF antibody is down regulated. (3) The VEGF content of SKOV3 in culture medium treated with anti VEGF antibody is lower than that in control ( P <0.05). Conclusions: VEGF antibody had no influence on growth of SKOV3 in vitro , but it could downregulate the expression of VEGF and it′s receptor and inhibit the secretion of VEGF in SKOV3.

关 键 词：血管内皮生长因子抗体 卵巢癌 血管内皮生长因子 体外实验 免疫组织化学 逆转录-聚合酶链反应 

分 类 号：R737.31[医药卫生—肿瘤]