IL—1及L—NMMA对兔髁状突软骨细胞NO生成和iNOS表达的影响  

Effects of IL - 1 and L - NMMA on the nitric oxide production and iNOS expression in rabbit TMJ chondrocytes.

作　　者：邹淑娟[1] 胡静[1] 高占巍[1] 王志国[1] 

机构地区：[1]四川大学华西口腔医学院,四川成都610041

出　　处：《临床口腔医学杂志》2002年第5期345-347,共3页Journal of Clinical Stomatology

摘　　要：目的 观察白细胞介素-1(IL-1)和L-单甲基精氨酸(L-NMMA)对兔髁状突软骨细胞一氧化氮(NO)合成和诱导型一氧化氮合酶(iNOS)基因表达的影响。方法 用硝酸还原酶法和RT-PCR反应分别检测软骨细胞培养上清液中NO浓度和细胞内iNOS mRNA表达的变化。结果 单独使用IL-1,软骨细胞培养液中NO水平和iNOS表达水平显著升高,配伍加入L-NMMA能有效降低由于IL-1刺激引起的NO水平升高,但不能降低iNOS表达的升高幅度。结论 IL-1能显著增加体外培养的兔髁状突软骨细胞iNOS的表达而促进NO的生成,而L-NMMA对这种效应有明显抑制作用。Objective To study the effects of IL - 1 and L - NMMA on the nitric oxide (NO) synthesis and iNOS gene expression in rabbit cultured TMJ chondrocytes. Methods NO accumulation in the cultured cell supernatant was measured with nitrate reductase method and the Griess reaction. RT - PCR reaction was used to detect the mRNA expression of iNOS. Results The significantly increased levels of NO production and iNOS expression were observed after IL- 1 application, but NO production can be reduced by addition of L - NMMA. Conclusion IL - 1 can increase NO production and expression of iNOS in rabbit TMJ chondrocytes in vitro, but this activity can be inhibited by L- NMMA.

关 键 词：髁状突 软骨细胞 一氧化氮 白细胞介素-1 L-单甲基精氨酸 颞下颌关节骨关节病 TMJOA 

分 类 号：R782.6[医药卫生—口腔医学]