8-MOP/UVA诱导体外培养真皮成纤维细胞线粒体DNA复制控制区点突变研究  

Study on point mutations in mitochondrial DNA control region for replication DLP_6 of cultured dermal fibroblast with 8-MOP/UVA treatment

作　　者：刘仲荣[1] 刘荣卿[1] 张国威[2] 郝飞[1] 阎衡[1] 

机构地区：[1]第三军医大学附属西南医院皮肤科 [2]第三军医大学附属新桥医院皮肤科,重庆400038

出　　处：《中华医学遗传学杂志》2002年第5期386-388,共3页Chinese Journal of Medical Genetics

摘　　要：目的　探讨皮肤光老化与真皮成纤维细胞线粒体 DNA复制控制区点突变的关系。方法　以8-甲氧补骨脂素 /长波紫外线 (8- methoxypsoralen / ultraviolet A,8- MOP/ UVA)作用体外培养的真皮成纤维细胞 ,一步法提取线粒体 DNA,PCR方法扩增线粒体 DNA复制控制区 D环及毗邻转录启动子区域片段 6 (D- loop and adjacent transcription promoter 6 ,DL P6 ) ,并以聚合酶链反应 -单链构象多态性及直接测序的方法检测 PCR产物的点突变。结果　 8- MOP/ UVA作用后 ,体外培养的真皮成纤维细胞线粒体 DNA复制控制区 DL P6 片段有大量 4 14 T→ G突变的累积。结论　真皮成纤维细胞线粒体 DNA复制控制区DL P6Objective To investigate the relationships between skin photoaging and point mutations in mitochondrial DNA (mtDNA) control region for replication of dermal fibroblast. Methods Cultured dermal fibroblasts were treated by 8 methoxypsoralen /ultraviolet A (8 MOP/UVA). mtDNA was extracted by one step method and the PCR products of D loop and adjacent transcription promoter 6 (DLP 6) fragment of mtDNA control region for replication were detected by polymerase chain reaction single strain conformation polymorphism and direct sequencing. Results After treated by 8 MOP/UVA, point mutations of 414 T→G of DLP 6 fragment of mtDNA control region for replication largely accumulated. Conclusion Accumulation of point mutations of DLP 6 fragment of mtDNA control region for replication may be closely associated with skin photoaging.

关 键 词：体外培养 点突变 成纤维细胞 皮肤光老化 线粒体DNA 8-甲氧补骨脂素 长波紫外线 

分 类 号：R751.02[医药卫生—皮肤病学与性病学]