大鼠正常肝脏和癌前病变肝脏微粒体谷胱甘肽 S-转移酶的纯化及其鉴定  

Purificaton and Identification of Microsomal Glutathione S-Traesferases in Normal and Preneoplastic Rat Liver

作　　者：金宗铉 尹宗柱[1] 林敬务 崔桂花 

机构地区：[1]延边医学院生物化学教研室 [2]延边医学院八五级药学系

出　　处：《延边大学医学学报》1990年第1期5-9,共5页Journal of Medical Science Yanbian University

摘　　要：本文通过 DE_(52)-纤维素和羟基磷灰石柱层析及 GSH-Sepharose-4B 亲和层析法,分离纯化了正常大鼠肝脏和癌前病变肝脏微粒体 GST,得到了三个微粒体 GST 同工酶组分,分别命名为A_1,A_2,A_3.用 SDS 聚丙烯酰胺凝胶电泳法测得其亚基分子量分别为25000D,26000D,43000D.以 CDNB 为底物按 Habig 方法测定 GST 活性,发现大鼠肝脏癌前病变微粒体同工酶 A_1和 A_2的活性显著增高,而同工酶 A_3在正常和癌前病变大鼠之间无显著差异.本实验结果表明,大鼠肝脏癌前病变组织的微粒体 GST 同工酶组分中 A_1和 A_2显著增高,可作为肝脏癌前病变的酶学指标.Normal glutathione S-transferase （GST） was purified from normal and hyperplastic nodule（HN） bearing rat liver by diethylaminoethyl cellulose and hydroxylapatite column chromatography followed by glutathione-Sepa- rose 4B affinity chromatography.Three isoenzymes of microsomal GST were separated and were named A₁,A₂ and A₃,respectively.The subunit molecular weights of three isoenzymes of microsomal GST determined by the sodium dodecyl sulfate polyacrylamide gel electrophoresis were 25kD, 26kD,and 43kD,respectively.Microsomal GST activities towards 1-chloro- 2,4-dinitrobenzene were determined by Habig’s method.It was found that the activities of isoenzyme A₁ and A₂ of microsomal GST in preneoplas- tic rat liver were markedly increased while As was changed a little in normal rat liver.The results indicate that microsomal GST isoenzyme A₁ and A₂ can be used as a preneoplastic hepatic marker enzyme.

关 键 词：谷胱甘肽转移酶 肝脏癌前病变 肝微粒体 

分 类 号：R[医药卫生]