带有白花甜菜染色体片段的栽培甜菜易位系的筛选  

SELECTION ON BETA VULGARIS L. TRANSLOCATION LINES WITH THE FRAGMENT OF CHROMOSOME FROM BETA COROLLIFLORA ZOSS

作　　者：韩晓云[1] 康传红[1] 郑天然[1] 郭德栋[1] 王桂芝[1] 

机构地区：[1]黑龙江大学生命科学学院,哈尔滨150080

出　　处：《植物研究》2002年第4期459-462,共4页Bulletin of Botanical Research

基　　金：国家 8 63计划 ( 863 -10 1-0 4-0 1-0 7)资助

摘　　要：本文采用斑点杂交方法 ,对近 1 0 0 %传递的甜菜单体附加系M1 4( 2n =1 9)发生分离的后代反转系 ( 2n =1 8)进行研究 ,采用随机引物法标记白花甜菜特异探针 ,对 80株反转系进行杂交实验 ,结果筛选出 1 9个易位系 ,这 1 9个易位系有待于进一步研究 。This paper reported the DNA Dot-blotting analyse on the progenies (2n=18),which seperated from M14(2n=19),the monosomic addition lines of Beta vulgaris with high transmission rate near100% The probe1054 was labeled with DIG Random Primed DNA Labeling method Among total 80 progenies,19 were proved to be translocation line with the fragment of chromosome from Beta corolliflora Further study are needed to confirm if these translocation lines have apomixis genes

关 键 词：白花 甜菜 染色体片段 易位系 筛选 无融合生殖基因 斑点杂交 

分 类 号：S566.3[农业科学—作物学]