鞘磷脂合成酶的提取和检测  

Extraction and Determination of Sphingomyelin Synthase

作　　者：江振然[1] 胡道伟[1] 柯铁[1] 梅兴国[1] 常俊丽[1] 

机构地区：[1]华中科技大学生命科学与技术学院,中国湖北武汉430074

出　　处：《生命科学研究》2002年第3期243-245,共3页Life Science Research

摘　　要：在 2 0℃条件下 ,采用相对饱和度为 80 %的 (NH4) 2 SO4一步盐析并经低温冷冻干燥 1 2h得到鞘磷脂合成酶粗酶粉后 。The sphingomyelin synthase was obtained by salting out in 80%(NH 4) 2SO 4 and dried in freeze drying conditions for 12?h.Some basic characteristics of this enzymatic reaction were studied.The enzyme was determined by HPLC.

关 键 词：鞘磷脂合成酶 提取 检测 高效液相色谱法 酶促反应 

分 类 号：Q555[生物学—生物化学]