温度对人肺癌细胞A549蛋白质表达的影响  

THE INFLUENCE OF TEMPERATURE ON THE PROTEIN EXPRESSION OF HUMAN LUNG CANCER CELL LINE A549

作　　者：陈子珺[1] 王三英[1] 陈晋安[1] 彭宣宪[1] 

机构地区：[1]厦门大学生命科学学院生物学系细胞生物学与肿瘤细胞工程教育部重点实验室,厦门361005

出　　处：《实验生物学报》2002年第3期179-179,共1页Acta Biologiae Experimentalis Sinica

基　　金：国家自然科学基金资助(30080029)

摘　　要：肺癌蛋白质组的研究,有助于阐明其发病机制并对肺癌的防治有益。本文从研究人肺癌细胞热休克蛋白的表达情况出发,通过比较37℃、42℃和45℃培养条件下的人肺癌细胞A549总蛋白质的双向电泳图谱,获得3个温度敏感的差异蛋白点,依次命名为P_1、P_2、P_3。对差异蛋白进行MALDI-TOF-MS分析和采用SWISS-PROT数据库中的Peptident软件检索后,初步鉴定P_1与2种醛酮还原酶家族成员相匹配,P_2可能为一种新蛋白,P_3为锌指蛋白11A。The study on proteome of human cancer is helpful to explain its pathogenesis and make good effect on its prevention and cure. We compared the 2-DE maps of whole proteins of human lung cancer line A549 at 37℃ , 42℃ and 45℃ for the purpose of studying the expression of its heat shock proteins. Three temperature-sensitive differential spots were obtained and named as P1,P2,P3, respectively. Analyzed by MALDI-TOF-MS and Peptident software searched in the SWISS-PROT database, the three differential proteins were elementarily identified. P; matches with two proteins belong to the Aldo-keto reductase family, P2 may be a new protein and P3 is Zinc finger protein 11A.

关 键 词：温度 人肺癌细胞A549 蛋白质 表达 肽质量指纹谱 基质辅助激光解吸/飞行时间质谱 热休克蛋白 

分 类 号：R734.2[医药卫生—肿瘤]